Enhancer control of transcriptional bursting

By Takashi Fukaya, Bomyi Lim, M. Levine. Cell, 2016

644 citations recorded by the source corpus.

Summary Transcription is episodic, consisting of a series of discontinuous bursts. Using live imaging methods and quantitative analysis, we examine transcriptional bursting in living Drosophila embryos. Different developmental enhancers positioned downstream of synthetic reporter genes produce transcriptional bursts with similar amplitudes and duration, but generate very different bursting frequencies, with strong enhancers producing more bursts than weak enhancers. Insertion of an insulator reduces the number of bursts and the corresponding level of gene expression, suggesting that enhancer regulation of bursting frequency is a key parameter of gene control in development. We also show that linked reporter genes exhibit coordinated bursting profiles when regulated by a shared enhancer, challenging conventional models of enhancer-promoter looping.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/ad1665a5a1529748ad5c9c5c1410eea5f3d8d26c

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In Molecular Biology

Citations in the corpus

Gene regulatory network inference in the era of single-cell multi-omics (2023, methodology): “To this end, super-resolution microscopy has also been used to validate candidate CRE–gene interactions 132 , although with less throughput than Hi-C.”
Ever-changing landscapes: transcriptional enhancers in development and evolution (2016, background): “Intriguingly, recent live-imaging studies in Drosophila uncovered the capacity of a single enhancer to drive synchronized transcriptional bursting from two equidistant promoters (Fukaya et al., 2016), raising the question of whether or how chromatin topology regulates this behavior.”
Liquid phase condensation in cell physiology and disease (2017, background): “It has become abundantly clear over the past decade that chromosomes and associated nuclear bodies are not randomly distributed in the nucleus (144), and nuclear architecture is intimately associated with dynamic gene regulation (144, 145).”