High-Throughput Mapping of a Dynamic Signaling Network in Mammalian Cells

By M. Barrios-Rodiles, K. Brown, B. Ozdamar, R. Bose, Zhongda C Liu, R. Donovan, F. Shinjo, Yongmei Liu, J. Dembowy, I. Taylor and 7 more. Science, 2005

776 citations recorded by the source corpus.

Subfields: Molecular Biology, Oncology

View Paper: https://www.semanticscholar.org/paper/5a880a5006b8b870307c45ae09bfa7115d14ee65

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. R. Weinberg
2. Jing Yang
3. A. Moustakas
4. C. Heldin
5. Trey Ideker
6. J. P. Thiery
7. Jormay Lim
8. René Bernards

In Oncology

1. R. Weinberg
2. Jing Yang
3. J. P. Thiery
4. Jormay Lim
5. A. Moustakas
6. C. Heldin
7. J. Sleeman
8. Trey Ideker

Citations in the corpus

• Differential network biology (2012, background): “In one early exception, Wrana and colleagues developed the LUMIER (luminescence-based mammalian interactome mapping) strategy to identify pairwise PPIs among a set of human factors with and without stimulation by transforming growth factor b (TGF b ) (Barrios-Rodiles et al , 2005).”
• Physiology and function of the tight junction. (2009, background): “An alternative role for occludin in coordinating transmembrane signaling is suggested by the findings that it binds the TGF-b type III receptor (Barrios-Rodiles et al. 2005) and manipulation of occludin in cultured cells affects Raf-1 signaling (Wang et al.”
• Tight junctions and cell polarity. (2006, background): “This may be important for the TGFβ-mediated epithelial-to-mesenchymal transition, which requires loss of polarity and dissolution of junctions (Barrios-Rodiles et al. 2005).”
• Large-scale mapping of human protein–protein interactions by mass spectrometry (2007, methodology): “Different techniques for mapping protein interactions, such as the yeast two-hybrid approach (Y2H) (Chien et al, 1991) and the LUMIER approach (Barrios-Rodiles et al, 2005), are available, and address the question of whether two proteins interact in a pairwise fashion.”
• Epithelial-mesenchymal transitions: insights from development (2012, background): “In invaginated mesodermal cells, the fibroblast growth factor (FGF) signaling components, Branchless (FGF), Htl (FGFR) and Stumps (Dof, FGFR-docker) are crucial for gastrulation (Beiman et al., 1996; Vincent et al., 1998; Stathopoulos et al., 2004; Kadam et al., 2009; Klingseisen et al., 2009).”
• An experimentally derived confidence score for binary protein-protein interactions (2008, neutral): “High-throughput yeast two-hybrid (Y2H) analyses identifies direct, binary interactions; other methods for identifying direct interactions have recently yielded smaller data set”
• Complex networks orchestrate epithelial–mesenchymal transitions (2006, background): “The dissolution of tight junctions is an early step during EMT, and recent high-throughput protein–protein-interaction screens have revealed the molecular mechanisms through which TGFβ signalling regulates this proces”
• TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal (2008, neutral): “Results TAZ interacts with Smad complexes To discover new effectors of the TGFβ signalling network we examined our LUMIER (for luminescence-based mammalian interaction mapping”
• Online Predicted Human Interaction Database (2005, neutral): “…the data within OPHID can be integrated with additional datasets ( e.g. , expression data from disease profiles, OMIM data on disease-related proteins) to reveal new protein interactions and pathways that may be involved in human disease (Barrios-Rodiles et al. , 2005; Seiden-Long et al. , 2005).”
• A highly conserved program of neuronal microexons is misregulated in autistic brains (2014, methodology): “To test this hypothesis, we employed luminescence-based mammalian interactome mapping (LUMIER; Barrios-Rodiles et al., 2005; Ellis et al., 2012) and coimmunoprecipitation-western blot assays to investigate whether the insertion of a highly conserved, neural-regulated 6 nt microexon in the nuclear…”