PCGF Homologs, CBX Proteins, and RYBP Define Functionally Distinct PRC1 Family Complexes

By Zhonghua Gao, Jin Zhang, R. Bonasio, Francesco Strino, A. Sawai, Fabio Parisi, Yuval Kluger, D. Reinberg. Molecules and Cells, 2012

857 citations recorded by the source corpus.

Summary The heterogeneous nature of mammalian PRC1 complexes has hindered our understanding of their biological functions. Here, we present a comprehensive proteomic and genomic analysis that uncovered six major groups of PRC1 complexes each containing a distinct PCGF subunit, a RING1A/B ubiquitin ligase, and a unique set of associated polypeptides. These PRC1 complexes differ in their genomic localization and only a small subset co-localize with H3K27me3. Further biochemical dissection revealed that the six PCGF-RING1A/B combinations form multiple complexes through association with RYBP or its homolog YAF2, which prevents the incorporation of other canonical PRC1 subunits such as CBX, PHC and SCM. Although both RYBP/YAF2- and CBX/PHC/SCM-containing complexes compact chromatin, only RYBP stimulates the activity of RING1B toward H2AK119ub1, suggesting a central role in PRC1 function. Knockdown of RYBP in ES cells compromised their ability to form embryoid bodies, likely because of defects in cell proliferation and maintenance of H2AK119ub1 level.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/5313305fd2291bc32189ab564b17d38cf1510c65

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In Molecular Biology

1. J. Simon
2. Robert E. Kingston
3. D. Reinberg
4. Luciano Di Croce
5. K. Helin
6. P. Voigt
7. Wee-Wei Tee
8. R. Klose

Citations in the corpus

• Occupying chromatin: Polycomb mechanisms for getting to genomic targets, stopping transcriptional traffic, and staying put (2013, background): “See (Gao et al., 2012; Gearhart et al., 2006; Lagarou et al., 2008; Tavares et al., 2012) for detailed descriptions of PRC1 variants.”
• A double take on bivalent promoters. (2013, background): “PRC1 forms several subcomplexes with unique subunit composition (Gao et al. 2012; Tavares et al. 2012; Simon and Kingston 2013).”
• Variant PRC1 Complex-Dependent H2A Ubiquitylation Drives PRC2 Recruitment and Polycomb Domain Formation (2014, background): “, 2002), while all PCGF proteins interact with RYBP/YAF2 to form variant PRC1 complexes lacking CBX proteins (Farcas et al., 2012; Gao et al., 2012; Gearhart et al., 2006; Lagarou et al., 2008; Sánchez et al., 2007; Tavares et al., 2012) (Figure 1A).”
• Transcriptional regulation by Polycomb group proteins (2013, background): “; and from the Novo Nordisk Foundation, the Danish Cancer Society and the Danish Council for Strategic Research (12-110503) to K.”