Variant PRC1 Complex-Dependent H2A Ubiquitylation Drives PRC2 Recruitment and Polycomb Domain Formation
By Neil P. Blackledge, A. Farcas, T. Kondo, Hamish W. King, J. McGouran, Lars L. P. Hanssen, Shinsuke Ito, Sarah Cooper, Kaori Kondo, Yoko Koseki and 7 more. Cell, 2014
724 citations recorded by the source corpus.
Summary Chromatin modifying activities inherent to polycomb repressive complexes PRC1 and PRC2 play an essential role in gene regulation, cellular differentiation, and development. However, the mechanisms by which these complexes recognize their target sites and function together to form repressive chromatin domains remain poorly understood. Recruitment of PRC1 to target sites has been proposed to occur through a hierarchical process, dependent on prior nucleation of PRC2 and placement of H3K27me3. Here, using a de novo targeting assay in mouse embryonic stem cells we unexpectedly discover that PRC1-dependent H2AK119ub1 leads to recruitment of PRC2 and H3K27me3 to effectively initiate a polycomb domain. This activity is restricted to variant PRC1 complexes, and genetic ablation experiments reveal that targeting of the variant PCGF1/PRC1 complex by KDM2B to CpG islands is required for normal polycomb domain formation and mouse development. These observations provide a surprising PRC1-dependent logic for PRC2 occupancy at target sites in vivo.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/c22acdb69efc4d395db3aac0ad04328b103fcb18
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Citations in the corpus
- The physics of epigenetics (2015, background): “However, recent studies showed that this relationship may also work in the opposite sense (Blackledge et al., 2014; Cooper et al., 2014).”
- Systematic discovery of Xist RNA binding proteins (2015, background): “This is consistent with recent reports demonstrating the PRC1-dependent recruitment of PRC2 complex (Blackledge et al., 2014; Cooper et al., 2014; Kalb et al., 2014).”
- Genome Regulation by Polycomb and Trithorax: 70 Years and Counting. (2017, positive): “Tethering KDM2B induces the recruitment of ncPRC1 complexes in a PRC2-independent manner, which results in the deposition of H2AK119ub and promotes binding of PRC2 (Blackledge et al., 2014; Cooper et al., 2014).”