Direct visualization of macrophage-assisted tumor cell intravasation in mammary tumors.

By J. Wyckoff, Yarong Wang, Elaine Lin, Jiu-feng Li, S. Goswami, E. Stanley, J. Segall, J. Pollard, J. Condeelis. Cancer Research, 2007

1,053 citations recorded by the source corpus.

Although the presence of macrophages in tumors has been correlated with poor prognosis, until now there was no direct observation of how macrophages are involved in hematogenous metastasis. In this study, we use multiphoton microscopy to show, for the first time, that tumor cell intravasation occurs in association with perivascular macrophages in mammary tumors. Furthermore, we show that perivascular macrophages of the mammary tumor are associated with tumor cell intravasation in the absence of local angiogenesis. These results show that the interaction between macrophages and tumor cells lying in close proximity defines a microenvironment that is directly involved in the intravasation of cancer cells in mammary tumors.

Subfields: Immunology, Molecular Biology, Oncology

View Paper: https://www.semanticscholar.org/paper/522fe9f2453f1dd4529a053788a11d7125c5e140

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Immunology

1. A. Mantovani
2. Lisa M. Coussens
3. J. Pollard
4. A. Sica
5. D. Hanahan
6. P. Allavena
7. J. Joyce
8. R. Weinberg

In Molecular Biology

1. R. Weinberg
2. Zena Werb
3. Lisa M. Coussens
4. M. Nieto
5. Pengfei Lu
6. D. Hanahan
7. A. Mantovani
8. M. Egeblad

In Oncology

1. R. Weinberg
2. A. Mantovani
3. Lisa M. Coussens
4. J. Pollard
5. D. Hanahan
6. Zena Werb
7. P. Allavena
8. J. Joyce

Citations in the corpus

• CD4+ T Cells Regulate Pulmonary Metastasis of Mammary Carcinomas by Enhancing Protumor Properties of Macrophages (2009, background): “This brief treatment resulted in a significant decrease in the number of circulating carcinoma cells present in PB (Figure 7C), similar to observations by Wyckoff et al. (2007).”
• Cancer-related inflammation (2008, background): “, with intravasation assisted by direct interactions between tumour cells and TAM”
• Tumor-associated macrophages in tumor metastasis: biological roles and clinical therapeutic applications (2019, methodology): “According to this experiment, an intravasating tumor cell is always visualized to be accompanied by a macrophage within one cell diameter, showing a direct evidence of TAMs involving in tumor cell intravasation [105, 106].”
• The CD47-signal regulatory protein alpha (SIRPa) interaction is a therapeutic target for human solid tumors (2012, background): “Circulating tumor cells may even be particularly vulnerable to anti-CD47 mAbs if they rely on transmitting a “don’t eat me” signal to perivascular macrophages for entry into blood vessels or tether to macrophages for assistance in migration (1, 42).”
• Epithelial-mesenchymal transitions in development and disease. (2009, background): “ing has also yielded evidence of EMT in cancer progression (Wyckoff et al., 2007).”
• Microenvironmental regulation of metastasis (2009, background): “TAMs also directly promote the process of cancer cell intravasation into the blood circulation, as shown by multiphoton intravital imaging of the breast microenvironmen”
• Visualizing stromal cell dynamics in different tumor microenvironments by spinning disk confocal microscopy (2008, positive): “, 2005), and distinguished between these cell types by the propensity of macrophages to take up injected fluorescent dextran (Wyckoff et al., 2007).”
• Protease-dependent versus -independent cancer cell invasion programs: three-dimensional amoeboid movement revisited (2009, background): “Given that only small numbers of carcinoma cells display rapid movement in vivo (Wyckoff et al., 2006, 2007; Xue et al., 2006; Sahai, 2007; Sahai et al., 2007), the issue arises as to the precise origin of this cell type.”