A bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors

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Impact of cytosine methylation on DNA binding specificities of human transcription factors (2017, methodology): “We (21, 26, 27) and others have previously established that motifs generated using HT-SELEX are similar to those obtained using other state-of-the-art methods, such as protein-binding microarrays or bacterial one-hybrid assays (28, 29), and are biologically relevant on the basis of their ability to ”
GLK Transcription Factors Coordinate Expression of the Photosynthetic Apparatus in Arabidopsis[W][OA] (2009, background): “Screens of a randomoligo-based library in a bacterial-1-hybrid system (Meng et al., 2005) revealed that while a GLK1 bait transactivates reporter constructs at a level significantly above background, no strong consensus sequence can be identified in recovered clones (data not shown).”
Transcriptional enhancers: from properties to genome-wide predictions (2014, methodology): “PBMs are highly dense arrays that are spotted with thousands of DNA molecules, which can be probed simultaneously for transcription factor binding; SELEX enriches transcription factor-bound sequences by repeated rounds of selection and sequencing; and Y1H and B1H assays convert transcription factor ”