Targeted genome modification of crop plants using a CRISPR-Cas system

By Qi-wei Shan, Yanpeng Wang, Jun Li, Yi Zhang, Kunling Chen, Zhen Liang, Kang Zhang, Jinxing Liu, J. Xi, J. Qiu and 1 more. Nature Biotechnology, 2013

1,788 citations recorded by the source corpus.

Subfields: Business and International Management, Insect Science, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/50c3b169e729a8f56e8d952a06b73d047cc06233

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In Business and International Management

1. Jin-Soo Kim
2. Daesik Kim
3. S. Cho
4. Jungeun Kim
5. Caixia Gao

In Insect Science

1. Caixia Gao
2. Jian‐Kang Zhu
3. Yong‐Bi Fu

In Molecular Biology

1. Caixia Gao
2. Jian‐Kang Zhu
3. Jin-Soo Kim
4. Sojung Kim
5. Kazuo Shinozaki
6. S. Cho
7. Daesik Kim
8. Lam-Son Phan Tran

Citations in the corpus

• Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System (2015, methodology): “(26), higher plants (27), Bombyx mori (28), Drosophila (29), and human cell lines (30–32).”
• Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation (2015, neutral): “system provides an alternative method [17–44].”
• Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins (2014, background): “2014) and plants (Li et al. 2013b; Nekrasov et al. 2013; Shan et al. 2013), rapidly catching up with their precursors, namely, zinc finger nucleases (ZFNs) (Bibikova et al.”
• CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences (2014, neutral): “These variants correspond to DNA bulges at positions 1, 2 or 3, 8, 9 or 10, 11, 18 and 19 from the PAM (Figure 2D).”
• The CRISPR/Cas9 system for plant genome editing and beyond. (2015, background): “…rice, when potential off-target sites with at least a conserved 12-nt seed sequence were investigated (Feng et al., 2014; Jia and Wang, 2014; Li et al., 2013; Nekrasov et al., 2013; Shan et al., 2013; Upadhyay et al., 2013; Zhou et al., 2014) even using whole-genome sequencing (Feng et al., 2014).”
• CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture. (2019, background): “In 2013, three independent groups established the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) system for use in rice (Oryza sativa), wheat (Triticum aestivum), Nicotiana benthamiana, and Arabidopsis thaliana (73, 115, 140).”
• A CRISPR/Cas9 toolkit for multiplex genome editing in plants (2014, methodology): “The CRISPR/Cas system has been harnessed to achieve efficient genome editing in a variety of organisms, including bacteria, yeast, plants, and animals, as well as human cell lines [12-27].”
• A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation1[OPEN] (2015, methodology): “To promote TALEN technology, we developed a streamlined TALEN assembly method (Cermak et al., 2011), which was later used for successful genome editing in many plant species (Christian et al., 2013; Shan et al., 2013a;Wendt et al., 2013; Zhang et al., 2013; Lor et al., 2014; Wang et al., 2014).”
• A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants. (2015, methodology): “Other Cas9 genes used for plant genome targeting (Li et al., 2013; Mao et al., 2013; Shan et al., 2013a, 2013b; Xie and Yang 2013; Zhou et al., 2014) lack such a feature of the 50 GC enrichment (Supplemental Figure 2).”