Subdiffraction Multicolor Imaging of the Nuclear Periphery with 3D Structured Illumination Microscopy

By Lothar Schermelleh, P. Carlton, S. Haase, L. Shao, Lukman Winoto, P. Kner, B. Burke, M. C. Cardoso, David A. Agard, Mats Gustafsson and 2 more. Science, 2008

1,140 citations recorded by the source corpus.

Subfields: Biophysics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/43f9394c7cf7b218ef18df1ba507978089bd0672

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Citations in the corpus

The conserved Bardet-Biedl Syndrome proteins assemble a coat that traffics membrane proteins to cilia (2010, neutral): “whose 300 nm diameter cannot be resolved by conventional light microscopy, we resorted to structured illumination microscopy, a ‘‘super-resolution’’ technique that lowers the optical resolution to less than 50 nm (Schermelleh et al., 2008).”
Modulation of protein properties in living cells using nanobodies (2010, background): “We have recently demonstrated that cellular structures can be imaged at subdiffraction resolution by three-dimensional structured illumination microscopy (3D-SIM”
Chromosome territories. (2010, background): “Recent developments of laser-based light microscopic techniques with ultra-high resolution has now opened possibilities for light optical nanoscopy, which will help to overcome these limitations (Hell 2007; Schermelleh et al 2008; Gunkel et al. 2009).”
A guide to super-resolution fluorescence microscopy (2010, methodology): “One such example is the identification of interchromatin channels leading up to individual nuclear pores (Schermelleh et al., 2008).”
Dynamics of DNA damage response proteins at DNA breaks: a focus on protein modifications. (2011, background): “…further progress in the field is also likely to be triggered by further technical improvements, such as new superresolution microscopes and associated computational tools that will allow hitherto unattainable resolution of DDR foci in both three-dimensional space and time (Schermelleh et al. 2008).”
Whole cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution (2008, neutral): “Structured illumination microscopy has been recently used to image multiple cellular components with a twofold improvement in 3D resolution compared to conventional fluorescence microscop”
Super resolution fluorescence microscopy (2009, background): “SSIM has only been demonstrated in two dimensions to date, while the nonsaturated SIM has provided ~100-nm lateral and ~300-nm axial resolution, allowing chromosomes and nuclear envelope structures to be better resolved compared to confocal microscopy (21).”