Cutting Edge: Repurification of Lipopolysaccharide Eliminates Signaling Through Both Human and Murine Toll-Like Receptor 21

By M. Hirschfeld, Ying Ma, J. Weis, S. Vogel, J. Weis. Journal of Immunology, 2000

1,297 citations recorded by the source corpus.

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

Mediators of innate immune recognition of bacteria concentrate in lipid rafts and facilitate lipopolysaccharide-induced cell activation. (2002, methodology): “…that impurities in the LPS preparation were responsible for the events observed, we repurified our commercial LPS using a protocol shown previously to remove contaminants from LPS preparations (Hirschfeld et al., 2000; Tapping et al., 2000) and used it to stimulate cells prior to raft isolation.”
Receptors, Mediators, and Mechanisms Involved in Bacterial Sepsis and Septic Shock (2003, background): “cells through TLR4 but lacked TLR2 binding (216).”
Variation in Microbiome LPS Immunogenicity Contributes to Autoimmunity in Humans. (2016, neutral): “LPS purification was performed by hot phenol-water method (Hirschfeld et al., 2000).”
Metabolic Induction of Trained Immunity through the Mevalonate Pathway. (2018, methodology): “Stimuli and pharmacological inhibitors used include BCG, IGF1, lipopolysaccharide (LPS, further purified as described (Hirschfeld et al., 2000)), Pam3Cys, PolyI:C, Macrophage-activating lipopeptide-2 (MALP2), Phytohemagglutinin (PHA), fluvastatin sodium hydrate, (R)-Mevalonic acid lithium salt,…”
Tumor necrosis factor receptor-associated factors (TRAFs) (2001, background): “TLR-4 is required for responsiveness to LPS (Hirschfeld et al., 2000).”