Sds3 (Suppressor of Defective Silencing 3) Is an Integral Component of the Yeast Sin3·Rpd3 Histone Deacetylase Complex and Is Required for Histone Deacetylase Activity*
By T. Lechner, M. Carrozza, Yaxin Yu, P. Grant, A. Eberharter, D. Vannier, G. Brosch, D. Stillman, D. Shore, Jerry L. Workman. Journal of Biological Chemistry, 2000
94 citations recorded by the source corpus.
SDS3 (suppressor of defective silencing 3) was originally identified in a screen for mutations that cause increased silencing of a crippled HMR silencer in arap1 mutant background. In addition, sds3mutants have phenotypes very similar to those seen in sin3and rpd3 mutants, suggesting that it functions in the same genetic pathway. In this manuscript we demonstrate that Sds3p is an integral subunit of a previously identified high molecular weight Rpd3p·Sin3p containing yeast histone deacetylase complex. By analyzing an sds3Δ strain we show that, in the absence of Sds3p, Sin3p can be chromatographically separated from Rpd3p, indicating that Sds3p promotes the integrity of the complex. Moreover, the remaining Rpd3p complex in the sds3Δ strain had little or no histone deacetylase activity. Thus, Sds3p plays important roles in the integrity and catalytic activity of the Rpd3p·Sin3p complex.
View Paper: https://www.semanticscholar.org/paper/4d9e89974cbfc4daaaf65ee8049f2ea18363d626
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- John R. Yates III
- Kenneth K. Lee
- M. Carrozza
- Bing Li
- L. Florens
- Jerry L. Workman
- Tamaki Suganuma
- S. Swanson
Citations in the corpus
- The SMRT and N-CoR Corepressors Are Activating Cofactors for Histone Deacetylase 3 (2001, background): “Gal4SMRT DAD mutants W432A, F440A, F451A, I454A, L458A, V463A, and Y470A were produced using the corresponding mutagenic oligonucleotides and the QuickChange site-directed mutagenesis kit (Stratagene).”
- Cotranscriptional set2 methylation of histone H3 lysine 36 recruits a repressive Rpd3 complex. (2005, unknown): “Five previously known Rpd3-interacting proteins were identified: Ume1, Sds3, Pho23, Sap30, and Cti6/Rxt1 (Kurdistani et al., 2002; Lechner et al., 2000; Loewith et al., 2001; Puig et al., 2004; Zhang et al., 1998) (Figure 1A).”
- Histone H3 methylation by Set2 directs deacetylation of coding regions by Rpd3S to suppress spurious intragenic transcription. (2005, methodology): “There are two known Rpd3 complexes within S. cerevisiae that were identified primarily by their gel filtration elution profiles (Kasten et al., 1997; Lechner et al., 2000; Rundlett et al., 1996).”