MethyLight: a high-throughput assay to measure DNA methylation.

By Cindy A. Eads, K. Danenberg, K. Kawakami, L. Saltz, Corey Blake, D. Shibata, P. Danenberg, Peter W. Laird. Nucleic Acids Research, 2000

1,459 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/4d61e1915832537eee9c5ea8ae4ec9a2f0f5d289

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Citations in the corpus

Cancer epigenomics: DNA methylomes and histone-modification maps (2007, methodology): “More recently, quantitative PCR-based methods, such as bisulphite treatment in combination with MethyLight”
CpG island hypermethylation and tumor suppressor genes: a booming present, a brighter future (2002, background): “Semiquantitative MethyLight data can be obtained by using a number of different probes for the sequence of interset.”
MGMT promoter methylation in malignant gliomas: ready for personalized medicine? (2010, methodology): “MSP can be performed using real-time PCR platforms that allow standardization, high-throughput analysis, and definition of cut-off points.(30,31)”
Identification of a CpG island methylator phenotype that defines a distinct subgroup of glioma. (2010, methodology): “MethyLight real-time PCR strategy was performed as described previously (Eads et al., 2000; Eads et al., 1999).”
High-throughput DNA methylation profiling using universal bead arrays. (2006, methodology): “…al. 1990), bisulfite DNA sequencing (Frommer et al. 1992; Dupont et al. 2004), methylation-specific PCR (MSP) (Herman et al. 1996) and MethyLight (Eads et al. 2000; Cottrell et al. 2004), methylation-sensitive single nucleotide primer extension (MS-SnuPE) (Gonzalgo and Jones 1997), restriction…”
Analysis of repetitive element DNA methylation by MethyLight (2005, methodology): “In this study, we advanced MethyLight assay technology, a quantitative, TaqMan-based real-time PCR system to analyze DNA methylation profiles (35), by extending it to the analysis of highly repeated DNA sequences.”
Liquid biopsy: a step closer to transform diagnosis, prognosis and future of cancer treatments (2022, background): “Fluorescence-based real-time modifications to conventional MSPs have also been shown to facilitate the quantitative detection of methylation patterns [130].”
DNA methylation in cancer: too much, but also too little (2002, methodology): “In collaboration with Peter Laird, we quantitatively analysed DNA from 31 Wilms tumors vs five types of normal tissues for hypermethylation at 13 CpG-rich regions by MethyLight, a bisulfite modification-dependent real-time PCR assay (Eads et al., 2000; Ehrlich et al., submitted).”
CpG island methylator phenotype, microsatellite instability, BRAF mutation and clinical outcome in colon cancer (2008, methodology): “Real-time PCR (MethyLight) for quantitative DNA methylation analysis Bisulfite treatment on genomic DNA and subsequent real-time PCR (MethyLight)[34] were validated and performed as previously described.”
Cancer epigenetics. (2005, positive): “Automated variants of MSP, such as the real-time PCR-based MethyLight technique (92), have both a sufficient signal-to-noise ratio and the throughput capacity to sensitively analyze a broad set of markers (82,93,94).”