Evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts.

By A. Klančnik, Saša Piskernik, B. Jeršek, S. Možina. Journal of Microbiological Methods, 2010

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Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants (2016, positive): “Microdilution and agar dilution are quantitative methods that can be used to determine MIC values (Kim et al. 2007).”
Antioxidant and Antimicrobial Activities of Thyme and Clove Essential Oils and Application in Minced Beef (2015, methodology): “Plates were incubated for 24 h and turbidity was determined by a microplate reader (Varioskan Flash, Thermo) at 600 nm with 30-min interval (Klančnik et al. 2010).”
Antibacterial Activity and Mode of Action of Ferulic and Gallic Acids Against Pathogenic Bacteria (2013, neutral): “6,31,35,65 All tests were performed in triplicate with three repeats.”
Phenolic Profile, Antioxidant Capacity, and Antimicrobial Activity of Leaf Extracts from Six Vitis vinifera L. Varieties (2013, methodology): “To indicate respiratory activity, the presence of the purple colour was determinated visually after adding 10 μL/well of INT (2- p -iodophenyl-3- p -nitrophenyl-5-phenyl tetrazolium chloride, Sigma) dissolved in water (2 mg/mL) and incubated at 37 °C for 30 min in dark ( 23 ).”