Nuclear Membrane Proteins with Potential Disease Links Found by Subtractive Proteomics

By E. C. Schirmer, L. Florens, T. Guan, John R. Yates III, L. Gerace. Science, 2003

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The Protein Composition of Mitotic Chromosomes Determined Using Multiclassifier Combinatorial Proteomics (2010, neutral): “Genuine components have been distinguished from contaminants in such preparations by subtractive (Schirmer et al., 2003) or quantitative (Foster et al., 2003) proteomics by determining the difference between two near-identical fractions, one enriched and the other depleted of thetargetstructure.”
The nuclear lamina comes of age (2005, background): “Also, the number of INM proteins that have been identified in mammalian cells is growing rapidl”
A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells (2012, background): “Proteomic screens have identi ed scores of membrane proteins that are enriched at the nuclear periphery (Schirmer et al., 2003).”
"Laminopathies": a wide spectrum of human diseases. (2007, background): “Approximately 80 proteins appear to be localized to the inner nuclear membrane in interphase mammalian cells [24].”
RNase P without RNA: identification and functional reconstitution of the human mitochondrial tRNA processing enzyme. (2008, methodology): “Other combinatorial proteomics approaches have previously been successfully used to define awkward biochemical preparations, and the PPOP principle can be viewed as a reversal of the concept of subtractive proteomics (Andersen et al., 2003; Schirmer et al., 2003).”