A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells

By K. Roux, D. I. Kim, M. Raida, B. Burke. Journal of Cell Biology, 2012

2,194 citations recorded by the source corpus.

Proximity-dependent biotin identification (BioID) is a new approach making use of biotin ligase fusion proteins for the identification of both interacting and neighboring proteins in their native cellular environment.

Subfields: Cell Biology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/d2064246d3ebc5d96eea800e0e62293bc8b470eb

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Citations in the corpus

An improved smaller biotin ligase for BioID proximity labeling (2016, methodology): “…applicability to insoluble proteins and weak/transient PPAs, the BioID method has rapidly become widely used to study PPAs in the nuclear envelope (Roux et al., 2012; Kim et al., 2014), centrosomes (Comartin et al., 2013; Firat-Karalar et al., 2014), cell–cell junctions (Van Itallie et al., 2013,…”
Site-specific biotinylation of purified proteins using BirA (2015, background): “Also the use of enzymes to achieve promiscuous biotinylation (a BirA mutant or a peroxidase) has enabled labeling of untagged proteins in particular cellular regions or compartments (30,31,32).”
Proteomic Mapping of Mitochondria in Living Cells via Spatially Restricted Enzymatic Tagging (2013, background): “One candidate is promiscuous biotin ligase (1–3), but its labeling kinetics are extremely slow (requiring 24 hours (1, 2); Fig.”
The BioGRID interaction database: 2015 update (2014, background): “For instance, a Proximity Label-Mass Spectrometry (MS) evidence code was recently introduced in order to document interactions detected upon covalent modification of interaction partners by diffusible reactive species produced by a bait-enzyme fusion protein (71).”
Multidimensional proteomics for cell biology (2015, methodology): “3b) , for example, on the basis of the ectopic expression of a protein of interest fused to either a promiscuous bio-tin-ligase derived from bacteria (the BioID method) 68 or to a peroxidase enzyme capable of activating biotin–phenol (the APEX method) 39 .”