Highly efficient endogenous human gene correction using designed zinc-finger nucleases

By F. Urnov, Jeffrey C. Miller, Ya-Li Lee, C. Beauséjour, Jeremy M. Rock, S. Augustus, A. Jamieson, M. Porteus, P. Gregory, M. Holmes. Nature, 2005

1,689 citations recorded by the source corpus.

Subfields: Molecular Biology, Oncology

View Paper: https://www.semanticscholar.org/paper/4828fda84d149846e9c896370bf86eff851eb176

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. D. Carroll
2. Jeffry D. Sander
3. J. Keith Joung
4. Daniel F. Voytas
5. Feng Zhang
6. A. Klug
7. Jin-Soo Kim
8. Nathan D. Lawson

In Oncology

1. S. Polo
2. Bonil Koo
3. Inha Heo
4. Stephen P. Jackson
5. Hans Clevers
6. M. Jasin
7. D. Hockemeyer
8. C. June

Citations in the corpus

• Zinc finger nuclease-mediated CCR5 knockout hematopoietic stem cell transplantation controls HIV-1 in vivo (2010, positive): “Since DSB formation requires only transient expression of ZFNs, gene delivery methods that are suitable to express ZFNs include plasmid DNA nucleofection, integrase-defective lentiviral vectors and adenoviral vectors (16,19,21).”
• Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases (2008, methodology): “In vertebrate cell lines, genomic lesions at a specific site have been introduced using zinc-finger nucleases (ZFNs”
• Targeting DNA Double-Strand Breaks with TAL Effector Nucleases (2010, background): “In theabsenceofa repair template, broken chromosomes are rejoined by nonhomologous end joining, which often introduces short DNA insertions or deletions that create targeted gene knockouts.”
• Rapid “open-source” engineering of customized zinc-finger nucleases for highly efficient gene modification (2008, positive): “…human cells (Alwin et al., 2005; Beumer et al., 2006; Bibikova et al., 2003; Bibikova et al., 2002; Lloyd et al., 2005; Lombardo et al., 2007; Miller et al., 2007; Moehle et al., 2007; Morton et al., 2006; Porteus and Baltimore, 2003; Szczepek et al., 2007; Urnov et al., 2005; Wright et al., 2005).”
• Efficient TALEN-mediated gene knockout in livestock (2012, background): “This finding has also been observed for cells treated with ZFN’s (11, 35, 36).”
• Engineering the Delivery System for CRISPR-based Genome Editing (2018, background): “By fusing these proteins to nucleases, it is possible to create a double-strand break at a specified site in the genome, which would then be repaired either through non-homologous end joining, usually leading to a small insertion or deletion and thus gene knockout, or homologous recombination with a”
• Distinct factors control histone variant H3.3 localization at specific genomic regions (2010, methodology): “, 2007) and correction (Urnov et al., 2005) modes to engineer a panel of heterozygous ES lines carrying one allele of wild-type H3.”