Efficient TALEN-mediated gene knockout in livestock

By D. F. Carlson, W. Tan, S. Lillico, Dana Štveráková, Chris Proudfoot, Michelle L. Christian, Daniel F. Voytas, C. Long, C. Whitelaw, S. Fahrenkrug. Proceedings of the National Academy of Sciences of the United States of America, 2012

602 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/47f94ce6954e3d2cc54089c1b97613539935a323

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Citations in the corpus

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases (2014, background): “Chromosomal DNA splicing induced by paired Cas9 nickases We and others have shown previously that two concurrent DSBs produced by engineered nucleases, such as ZFNs and TALENs, can promote large deletions of the intervening chromosomal segments (Carlson et al. 2012).”
Repair of strand breaks by homologous recombination. (2013, background): “These “designer” nucleases are being used for genome engineering in a variety of organisms in addition to mammalian cells, including zebrafish (Hwang et al. 2013) and livestock (Carlson et al. 2012).”
TALENs: a widely applicable technology for targeted genome editing (2012, positive): “Most of these studies used a single TALEN pair to generate NHEJ-induced knockout mutations but two of these reports also described the use of two TALEN pairs targeted to the same chromosome to generate deletions and/or inversions of large chromosomal segments 14, 17 .”
Applications of genome editing technology in the targeted therapy of human diseases: mechanisms, advances and prospects (2020, methodology): “In 2012, Carlson et al.(112) used TALEN technology to target LDLR in porcine fetal fibroblasts and obtained miniature swine containing mono- and biallelic mutations in LDLR, thus generating models of familial hypercholesterolemia, which came with critical biomedical significance in simulating lipid ”
Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice (2013, background): “knockout of the CCR5 gene for HIV resistance in human cells (21); disruption of the LDL receptor in swine (22); destruction of the bacterial blight disease susceptibility gene in rice (23); replacement of a tyrosine hydroxylase gene via TALEN-enhanced homologous recombination in zebrafish (24,25)].”
Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins (2014, background): “…induced by RGEN RNPs We and others have shown that the repair of two concurrent DSBs produced by ZFNs and TALENs gives rise to targeted chromosomal rearrangements such as deletions, inversions, and translocations (Brunet et al. 2009; Lee et al. 2010, 2012; Carlson et al. 2012; Kim et al. 2013a).”
Repurposing the CRISPR-Cas9 system for targeted DNA methylation (2016, background): “Development of genome editing tools such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and the CRISPR-Cas9 system (1–3) has opened new possibilities for analyses of gene function by targeting and altering almost any sequence in the genome of cultured cells”
Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair (2014, background): “Inducing DSBs with TALENs or RGNs at two sites on a chromosome can be used to trigger chromosomal deletions and inversions in cultured cells and zebrafish (Carlson et al. 2012; Gupta et al. 2013; Lim et al. 2013; Xiao et al. 2013).”