MicroRNA-143 Regulates Adipocyte Differentiation*

By Christine Esau, Xiaolin Kang, Eigen Peralta, E. Hanson, E. Marcusson, L. V. Ravichandran, Yingqing Sun, S. Koo, Ranjan J. Perera, Ravi Jain and 5 more. Journal of Biological Chemistry, 2004

1,027 citations recorded by the source corpus.

MicroRNAs (miRNAs) are endogenously expressed 20-24 nucleotide RNAs thought to repress protein translation through binding to a target mRNA (1-3). Only a few of the more than 250 predicted human miRNAs have been assigned any biological function. In an effort to uncover miRNAs important during adipocyte differentiation, antisense oligonucleotides (ASOs) targeting 86 human miRNAs were transfected into cultured human pre-adipocytes, and their ability to modulate adipocyte differentiation was evaluated. Expression of 254 miRNAs in differentiating adipocytes was also examined on a miRNA microarray. Here we report that the combination of expression data and functional assay results identified a role for miR-143 in adipocyte differentiation. miR-143 levels increased in differentiating adipocytes, and inhibition of miR-143 effectively inhibited adipocyte differentiation. In addition, protein levels of the proposed miR-143 target ERK5 (4) were higher in ASO-treated adipocytes. These results demonstrate that miR-143 is involved in adipocyte differentiation and may act through target gene ERK5.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/457580c663b98aa1011e60f1fb46199207bc300a

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cancer Research

In Molecular Biology

Citations in the corpus

miR-122 regulation of lipid metabolism revealed by in vivo antisense targeting. (2006, background): “A potential role for miRNAs in metabolic pathways has been suggested by studies implicating miRNAs in regulation of adipocyte differentiation (Esau et al., 2004) and glucose-stimulated insulin secretion from pancreatic islet cells (Poy et al.”
Oncomirs — microRNAs with a role in cancer (2006, background): “; miR-143 during mammalian adipocyte differentiatio”
Detection and characterization of placental microRNAs in maternal plasma. (2008, background): “miRNAs are short (19–25 nucleotides), single-stranded, and nonprotein-coding RNAs (14–16) that regulate gene expression by binding to the 3 untranslated region of the target mRNAs (17) and function in diverse biological processes, including development (18), differentiation ( 19 ), apoptosis (20), a”
Comprehensive analysis of microRNA expression patterns in hepatocellular carcinoma and non-tumorous tissues (2006, background): “The biological functions and targets of some miRNAs have been recently reported. miR-143 has been associated with adipocyte differentiation and the target of this miRNA is ERK5 (Esau et al., 2004). miR-375 regulates insulin secretion by modulating the expression of myotropin (Poy et al., 2004).”
NONALCOHOLIC STEATOHEPATITIS IS ASSOCIATED WITH ALTERED HEPATIC MICRO RNA EXPRESSION (2008, neutral): “Transcription factors Homeobox protein (HOX) 146b, 199a*, 455, 128b, 128a, 127, 214 188, 191*, 139, 145, 92b, 99b, 181b, 27b, 100, 16, 23a, 23b, 125b 122, 26b Sex-determining region Y-box (SOX) 224, 34a, 200a, 199a*, 128b, 214, 99b, 28, 126 214, 99b, 181b, 24, 27b, 23a, 23b, 125b 188, 145, 203, 92, ”
MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells. (2005, background): “In mammals, functions for specific miRNAs have been described in the processes of hematopoietic (6) and adipocyte differentiation (7) and insulin secretion (8).”