3'-minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures.
By I. Kutyavin, I. Afonina, A. Mills, V. Gorn, E. Lukhtanov, E. Belousov, M. J. Singer, D. Walburger, S. Lokhov, A. Gall and 4 more. Nucleic Acids Research, 2000
847 citations recorded by the source corpus.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/44f37338054b08aa1cfe0715436bc7785a62a175
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Citations in the corpus
- The real-time polymerase chain reaction. (2006, background): “MGBprobes are hydrolysis probes with a minor groove binding molecule attached to the end of the probe to increase affinity for DNA, which makes it possible to use shorter probes (Kutyavin et al., 2000).”
- Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. (2002, background): “Fluorescence quenching is more efficient, giving increased sensitivity (Kutyavin et al. 2000) and the higher melting temperatures (Tm) allows the design of significantly shorter probes that are also more specific, especially if there is a mismatch in the MGB region of the duplex.”
- Real-time PCR in the microbiology laboratory. (2004, methodology): “This chemistry, commercially called the Eclipse oligoprobes, replaces the TaqMan oligoprobe’s standard TAMRA quencher with a proprietary NFQ and incorporates a molecule that hyperstabilises the oligoprobe–target duplex by folding into the minor groove of the dsDNA [65,66].”
- Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods. (2000, methodology): “The 39 minor groove binder ligand allows use of shorter probes with better mismatch discrimination and lower fluorescent background (14).”
- Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology. (2009, background): “More recent advances in TaqMan probe technology have involved the introduction of the minor groove binder (MGB) probe (Kutyavin et al., 2000).”
- Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. (2002, methodology): “A novel type of TaqMan probe is available from Applied Biosystems which uses a “minor groove binding” (MGB) moiety on the 3 end that raises the effective melting temperature (Tm) of the probe, thereby enabling the probe to be significantly shorter and enhancing the function in an allelic discriminat”