Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology.

By Cindy J. Smith, Osborn, M. Osborn, Michael Wagner. FEMS Microbiology Ecology, 2009

889 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/66311c6d1c63de323384345e9f47097b9fb0820e

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In Ecology

In Molecular Biology

Citations in the corpus

Using microbial communities and extracellular enzymes to link soil organic matter characteristics to greenhouse gas production in a tidal freshwater wetland (2014, methodology): “Quantitative polymerase chain reaction (qPCR) assays were performed to assess the genetic potential of the microbial communities (Smith and Osborn 2009).”
Molecular Techniques to Assess Microbial Community Structure, Function, and Dynamics in the Environment (2011, methodology): “markers (Bustin et al. 2005; Smith and Osborn 2009).”
Effect of dietary components on the gut microbiota of aquatic animals. A never‐ending story? (2016, methodology): “RT-PCR is, however, a promising method for investigating bacterial processes in environmental samples, but the limitations, such as methods for high-quality RNA isolation and a prior knowledge of the target sequences, should be noticed (Smith & Osborn 2009).”
A method for simultaneous measurement of soil bacterial abundances and community composition via 16S rRNA gene sequencing (2016, background): “If the objective is to determine absolute abundances of a certain taxon in a sample, quantitative PCR with taxon-specific primers is the advised method for now, even though these abundance estimates can also be influenced by differences in amplification efficiencies, choice of primers, DNA extractio”
Unifying the analysis of high-throughput sequencing datasets: characterizing RNA-seq, 16S rRNA gene sequencing and selective growth experiments by compositional data analysis (2014, background): “Some groups additionally use quantitative PCR approaches to normalize reads [6].”