Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods.

By Thomas D. Schmittgen, B. A. Zakrajsek, A. Mills, V. Gorn, M. J. Singer, M. Reed. Analytical Biochemistry, 2000

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Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. (2002, methodology): “Today, the chemistries most commonly employed include 5 nuclease assays using TaqMan probes [2,3], molecular beacons [4], and SYBR Green I intercalating dyes [5,6] (Fig.”
Experimental validation of novel and conventional approaches to quantitative real-time PCR data analysis. (2003, background): “This compares favourably with previous calculations of real-time variability, of 14.2% for Sybr Green and 24.0% for TaqMan‘ ( 19 ), and furthermore suggests that intra-assay variability may be both primer and template dependent.”