Membrane budding and scission by the ESCRT machinery: it's all in the neck
By James H. Hurley, P. Hanson. Nature reviews. Molecular cell biology, 2010
733 citations recorded by the source corpus.
The endosomal sorting complexes required for transport (ESCRTs) catalyse one of the most unusual membrane remodelling events in cell biology. ESCRT-I and ESCRT-II direct membrane budding away from the cytosol by stabilizing bud necks without coating the buds and without being consumed in the buds. ESCRT-III cleaves the bud necks from their cytosolic faces. ESCRT-III-mediated membrane neck cleavage is crucial for many processes, including the biogenesis of multivesicular bodies, viral budding, cytokinesis and, probably, autophagy. Recent studies of ultrastructures induced by ESCRT-III overexpression in cells and the in vitro reconstitution of the budding and scission reactions have led to breakthroughs in understanding these remarkable membrane reactions.
Subfields: Cell Biology, Physiology
View Paper: https://www.semanticscholar.org/paper/446df0bcc6f6a10ca2fa744f358da480454c3757
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Citations in the corpus
- Membrane Vesicle Release in Bacteria, Eukaryotes, and Archaea: a Conserved yet Underappreciated Aspect of Microbial Life (2012, background): “fungi, and archaea (48) (a full review of this machinery can be found elsewhere 237 (26, 32, 98)).”
- Endosome maturation (2011, background): “Since the ESCRT machinery and its functions has been reviewed on many occasions (Babst, 2005; Eden et al, 2009; Raiborg and Stenmark, 2009; Woodman, 2009; Hurley, 2010; Hurley and Hanson, 2010; Roxrud et al, 2010), we will only discuss some of the general aspects.”
- Biogenesis of extracellular vesicles (EV): exosomes, microvesicles, retrovirus-like vesicles, and apoptotic bodies (2013, unknown): “In this regard, it is not surprising that certain proteins involved in EV formation also participate in cytokinesis [40].”
- Exosomes: key players in cancer and potential therapeutic strategy (2020, background): “During the process of MVBs biogenesis, the ESCRT-0 complex is recruited by ubiquitinated cargo, the major pathway-specific signal in MVBs biogenesis, to the endosomal membrane; the ESCRT-I and -II components make membrane deformation into buds and isolate the payload; and the ESCRT-III complex separ”
- Extracellular vesicle-based therapeutics: natural versus engineered targeting and trafficking (2019, background): “the expression of lysosome-associated membrane protein 2 fusion proteins(38) (8), glycosylphosphatidylinositol-anchored targeting moieties(81) (9), and transferrin-conjugated magnetic particles bound to transferrin receptor expressed on EVs(93) (10) Murphy et al.”
- Membrane curvature at a glance (2015, background): “Membrane scission is a key step in the budding of coated vesicles from the ER (COPII coat), Golgi (COPI coat), trans-Golgi network (AP-1 and AP-3 clathrin coats), endosomes (ESCRT complexes) and plasma membranes (AP-2 clathrin coat), as well as in virus budding (Ferguson and De Camilli, 2012; Hurley”
- Syndecan–syntenin–ALIX regulates the biogenesis of exosomes (2012, background): “Note that truncated forms of Alix containing the V domain interact with the two parts of the N-Ter domain of syntenin that contain LYPXL motifs, (1-30) and (31-60), and that mutation of the three LYPXL motifs (YP to AA) abolishes the interaction.”
- Analysis of ESCRT functions in exosome biogenesis, composition and secretion highlights the heterogeneity of extracellular vesicles (2013, background): “…and sequesters ubiquitylated proteins in the endosomal membrane, whereas the ESCRT-I and -II complexes appear to be responsible for membrane deformation into buds with sequestered cargo, and ESCRT-III components subsequently drive vesicle scission (Hurley and Hanson, 2010; Wollert et al., 2009).”