Isolation and in vitro expansion of human colonic stem cells

By P. Jung, Toshiro Sato, A. Merlos-Suárez, Francisco M. Barriga, M. Iglesias, David Rossell, Herbert Auer, M. Gallardo, M. Blasco, E. Sancho and 2 more. Nature Medicine, 2011

693 citations recorded by the source corpus.

Subfields: Cellular and Molecular Neuroscience, Molecular Biology, Oncology

View Paper: https://www.semanticscholar.org/paper/43c24bea3ac5d0fe242c2b3fcee349b7cd99d3bd

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cellular and Molecular Neuroscience

1. R. Nusse
2. Robert Langer
3. Hans Clevers
4. J. Massagué

In Molecular Biology

1. Hans Clevers
2. R. Nusse
3. E. Batlle
4. J. Massagué
5. M. van de Wetering
6. H. Begthel
7. X. Zhang
8. H. Gehart

In Oncology

1. Hans Clevers
2. J. Massagué
3. E. Batlle
4. R. Nusse
5. X. Zhang
6. M. van de Wetering
7. H. Begthel
8. H. Gehart

Citations in the corpus

• Stem Cell Organoid Engineering (2016, positive): “…human intestinal organoid culture, Wnt proteins and other factors (e.g., Tgf-β inhibitor, p38 inhibitor, Nicotinamide, etc.) are added, which essentially prevents the differentiation of the stem cells in culture, resulting in reduced diversity of cell types (Jung et al., 2011; Sato et al., 2011a).”
• Dependency of colorectal cancer on a TGF-beta-driven programme in stromal cells for metastasis initiation (2012, methodology): “We isolated EPHB2-high CoCSCs from the primary tumor of a patient with stage IV CRC and cultured them in conditions similar to those used for expansion of normal colon stem cells (Jung et al., 2011; Sato et al., 2011).”
• Wnt/ b -Catenin Signaling, Disease, and Emerging Therapeutic Modalities (2017, background): “Addition of small molecule inhibitors of Alk and p38 allowed long-term culture of human small intestine and colon organoids (Jung et al., 2011; Sato et al., 2011).”
• Modeling Development and Disease with Organoids. (2016, background): “, 2011), EphB2 (Jung et al., 2011), and CD166+/GRP78 (Wang et al.”
• Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver (2015, positive): “We have recently adapted the technology to allow culturing of human intestinal stem cells (Jung et al., 2011; Sato et al., 2011) and have shown that patient-derived intestinal organoids recapitulate the pathology of hereditary intestinal diseases (Bigorgne et al., 2014; Dekkers et al., 2013;…”
• Identification of multipotent luminal progenitor cells in human prostate organoid cultures (2014, background): “Fibroblast growth factor-10 (FGF10), FGF2, and prostaglandin E2 (PGE2) have been shown to support proliferation of prostate cell lines and/or human colon epithelium (Jung et al, 2011; Memarzadeh et al., 2007).”
• Functional repair of CFTR by CRISPR/Cas9 in intestinal stem cell organoids of cystic fibrosis patients. (2013, background): “As human intestinal stem cells in culture require additional Wnt for self-renewal and expansion (Jung et al., 2011; Sato et al., 2011), transfected stem cells were seeded in medium lacking both Wnt and R-spondin.”