Modulation of gut microbiota during probiotic-mediated attenuation of metabolic syndrome in high fat diet-fed mice
By Jingjing Wang, Huang Tang, Chenhong Zhang, Yufeng Zhao, M. Derrien, E. Rocher, Johan ET van-Hylckama Vlieg, K. Strissel, Liping Zhao, M. Obin and 1 more. The ISME Journal, 2014
766 citations recorded by the source corpus.
Structural disruption of gut microbiota and associated inflammation are considered important etiological factors in high fat diet (HFD)-induced metabolic syndrome (MS). Three candidate probiotic strains, Lactobacillus paracasei CNCM I-4270 (LC), L. rhamnosus I-3690 (LR) and Bifidobacterium animalis subsp. lactis I-2494 (BA), were individually administered to HFD-fed mice (108 cells day−1) for 12 weeks. Each strain attenuated weight gain and macrophage infiltration into epididymal adipose tissue and markedly improved glucose–insulin homeostasis and hepatic steatosis. Weighted UniFrac principal coordinate analysis based on 454 pyrosequencing of fecal bacterial 16S rRNA genes showed that the probiotic strains shifted the overall structure of the HFD-disrupted gut microbiota toward that of lean mice fed a normal (chow) diet. Redundancy analysis revealed that abundances of 83 operational taxonomic units (OTUs) were altered by probiotics. Forty-nine altered OTUs were significantly correlated with one or more host MS parameters and were designated ‘functionally relevant phylotypes’. Thirteen of the 15 functionally relevant OTUs that were negatively correlated with MS phenotypes were promoted, and 26 of the 34 functionally relevant OTUs that were positively correlated with MS were reduced by at least one of the probiotics, but each strain changed a distinct set of functionally relevant OTUs. LC and LR increased cecal acetate but did not affect circulating lipopolysaccharide-binding protein; in contrast, BA did not increase acetate but significantly decreased adipose and hepatic tumor necrosis factor-α gene expression. These results suggest that Lactobacillus and Bifidobacterium differentially attenuate obesity comorbidities in part through strain-specific impacts on MS-associated phylotypes of gut microbiota in mice.
Subfields: Infectious Diseases, Molecular Biology, Physiology
View Paper: https://www.semanticscholar.org/paper/43b07b8cc83fde2247b35cf239a156dda66de373
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Molecular Biology
Citations in the corpus
- Bacillus As Potential Probiotics: Status, Concerns, and Future Perspectives (2017, background): “It has been clearly understood that the gut inhabitants and their proper balance is the foremost criteria that determines healthy status, particularly in terms of metabolic disorders (Wang et al., 2015).”
- Gut Microbiota and Immune System Interactions (2020, background): “It has been suggested that gut dysbiosis may induce immune dysregulation and subsequently increase the risk of evelo ing diseas s, including inflamm tory bowel disease (IBD), diabetes, ca diovascular diseases (CVDs), and autoimmune dis as (Figure 3) [81,82].”
- Review: Diversity of Microorganisms in Global Fermented Foods and Beverages (2016, background): ““Functionally relevant phylotypes” in an ecosystemmay be specifically detected by, e.g., weighted UniFrac principal coordinate analysis based on 454 pyrosequencing of 16S rRNA genes, as applied in studies on gut microbiota (Wang et al., 2015).”