Glioblastoma microvesicles transport RNA and protein that promote tumor growth and provide diagnostic biomarkers
By J. Skog, T. Wurdinger, Sjoerd van Rijn, D. Meijer, Laura Gainche, M. Sena-Esteves, W. Curry, R. Carter, Anna M. Krichevsky, X. Breakefield. Nature Cell Biology, 2008
2,814 citations recorded by the source corpus.
Glioblastoma tumour cells release microvesicles (exosomes) containing mRNA, miRNA and angiogenic proteins. These microvesicles are taken up by normal host cells, such as brain microvascular endothelial cells. By incorporating an mRNA for a reporter protein into these microvesicles, we demonstrate that messages delivered by microvesicles are translated by recipient cells. These microvesicles are also enriched in angiogenic proteins and stimulate tubule formation by endothelial cells. Tumour-derived microvesicles therefore serve as a means of delivering genetic information and proteins to recipient cells in the tumour environment. Glioblastoma microvesicles also stimulated proliferation of a human glioma cell line, indicating a self-promoting aspect. Messenger RNA mutant/variants and miRNAs characteristic of gliomas could be detected in serum microvesicles of glioblastoma patients. The tumour-specific EGFRvIII was detected in serum microvesicles from 7 out of 25 glioblastoma patients. Thus, tumour-derived microvesicles may provide diagnostic information and aid in therapeutic decisions for cancer patients through a blood test.
Subfields: Cancer Research, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/41d29e52491d767f1d909b95196f92103844a359
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Cancer Research
- C. Théry
- Raghu Kalluri
- G. Raposo
- Suresh Mathivanan
- Hina Kalra
- X. Breakefield
- Richard J. Simpson
- A. Llorente
In Molecular Biology
- C. Théry
- Raghu Kalluri
- G. Raposo
- Suresh Mathivanan
- Richard J. Simpson
- X. Breakefield
- Hina Kalra
- A. Llorente
Citations in the corpus
- Vesiclepedia: A Compendium for Extracellular Vesicles with Continuous Community Annotation (2012, background): “Recent studies have highlighted the role of EVs in intercellular communication [20–22], vaccine and drug delivery [23– 25], and suggested a potential role in gene vector therapy [26] and as disease biomarkers [27].”
- Therapeutically harnessing extracellular vesicles (2022, background): “Three main approaches to therapeutically target EVs are being explored: (1) inhibiting EV release by targeting the neutral sphingomyelinase (nSMase 2) pathway, (2) inhibiting EV release by targeting components of the ESCRT machinery and (3) inhibiting uptake of pathological EVs by recipient cells.”
- Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes (2011, background): “The concept of using exosomes for gene delivery was previously suggested in two reports demonstrating transport of endogenous mRNAs and microRNAs expressed in the exosome-producing cells to other cells in cultur”
- miR-21: a small multi-faceted RNA (2008, background): “Journal compilation © 2009 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd exosomes [107].”
- Exosomes and their roles in immune regulation and cancer. (2015, background): “[100] Skog J, Wurdinger T, van Rijn S, Meijer DH, Gainche L, Sena-Esteves M, et al.”
- The biology, function, and biomedical applications of exosomes (2020, positive): “Exogenous bolus doses of supraphysiological levels of exosomes into mice were associated with a penetrant cellular phenotype, includingmodulation of cancer progression (144, 151, 165, 235), induction of neoplasia (148), and regeneration of tissue (236).”