Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral.

By G. Baird, D. Zacharias, Roger Tsien. Proceedings of the National Academy of Sciences of the United States of America, 2000

945 citations recorded by the source corpus.

Subfields: Biophysics, Ecology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/3c854cb79441081c252970a557f52daf456b1359

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Biophysics

In Ecology

In Molecular Biology

Citations in the corpus

A global double‐fluorescent Cre reporter mouse (2007, methodology): “DsRed, derived from coral Discosoma sp., was one of the first cloned RFPs and an excellent tool for in vitro studies (Baird et al., 2000; Matz et al., 1999).”
Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed) (2002, background): “Biophysical and X-ray crystallographic studies revealed that DsRed forms a stable tetramer, and that each monomer is structurally very similar to GFP (ref”
Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation[OPEN] (2016, methodology): “…to 59orientation going toward theT-DNA rightborder) andamaizeUbiquitin (Ubi ) promoter andUbi intron driving expression of aDsRED coding sequence (Baird et al., 2000) with an introduced potato LS1 intron (Vancanneyt et al., 1990). pPHP32269-LBA (Supplemental Figure 1B) contained two expression…”
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein (2004, background): “Unfortunately, all wild-type yellow-to-red fluorescent proteins reported so far are obligately tetrameric and often toxic or disruptiv”
Diversity and Evolution of Coral Fluorescent Proteins (2008, methodology): “According to the conservative method of semi-native gel electrophoresis [26], all the newly cloned FPs are tetramers or higher-order oligomers.”
A versatile toolbox for PCR‐based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes (2004, background): “The red fluorescent protein DsRed has been limited to special application in yeast (Pereira et al., 2001), since the formation of the red chromophore (Baird et al., 2000) is not fast enough (T1/2 ∼ 24 h) to allow the detection of de novo synthesized proteins in logarithmically growing cells.”
Fluorescent proteins and their applications in imaging living cells and tissues. (2010, background): “ble homotetramers even at very low (nanomolar) concentrations (26).”
Two-photon absorption properties of fluorescent proteins (2011, neutral): “5 [78] [28] [44] [41] [44] [45] [46] [23] [47] [48] [38] [49] [50] [51] [34] [43] mStrawberry(41) 596 596 576 574 2.”
Dissecting tumour pathophysiology using intravital microscopy (2002, background): “With the availability of live reporters that change 'colour' (wavelength of peak emission) with tim”
Scleractinian corals with photoprotective host pigments are hypersensitive to thermal bleaching (2004, background): “…identity have also demonstrated that only cultured clade type A symbionts (from high-light specializing corals collected in the Caribbean; Rowan et al. 1997) produced significant quantities of the UV absorbing mycosporine-like amino acids (MAAs) under experimental conditions (Banaszak et al. 2000).”