Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein

By Nathan Christopher Shaner, Robert E. Campbell, Paul A. Steinbach, B. Giepmans, A. Palmer, Roger Tsien. Nature Biotechnology, 2004

4,789 citations recorded by the source corpus.

Subfields: Biophysics, Molecular Biology, Organic Chemistry

View Paper: https://www.semanticscholar.org/paper/4ce1aba88ba4b6bd4ed1996cf4000f40df805c1e

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In Biophysics

1. Sergey A. Lukyanov
2. Konstantin A. Lukyanov
3. Dmitriy M. Chudakov
4. S. Muyldermans
5. G. Kremers
6. Michael W. Davidson
7. M. Matz
8. David W. Piston

In Molecular Biology

1. D. Klionsky
2. Beth Levine
3. N. Mizushima
4. V. Deretic
5. Guido Kroemer
6. S. Muyldermans
7. Alec C. Kimmelman
8. Sergey A. Lukyanov

In Organic Chemistry

1. Carolyn R. Bertozzi
2. Jennifer A. Prescher
3. Paul A. Steinbach
4. Roger Tsien
5. Philip A. Romero
6. D. Klionsky
7. Frances H. Arnold
8. Michael W. Davidson

Citations in the corpus

• A Guide to Fluorescent Protein FRET Pairs (2016, background): “Thus far, only a few truly monomeric RFPs are reported: mFruits from DsRed [110], mRuby derivatives from eqFP611 [111], and FusionRed from eqFP578 [112].”
• Fluorescent protein FRET: the good, the bad and the ugly. (2007, background): “Other pairs include GFP or YFP as the donor coupled with orange or red derivatives such as mKO [37] or mCherry [ 38 ], and an orange donor coupled with a red acceptor.”
• Germinal Center Dynamics Revealed by Multiphoton Microscopy Using a Photoactivatable Fluorescent Reporter (2010, neutral): “The GC light zone was identified by injecting NP conjugated to red fluorescent protein tdTomato (Shaner et al., 2004) (NP-tdTomato), which forms immune complexes that bind to FDCs in NP-immunized mice (Fooksman et al., 2010).”
• Exploring protein fitness landscapes by directed evolution (2009, positive): “Directed evolution had a key role by improving many features of fluorescent proteins, including emission and excitation properties, quantum yield, multimerization state and maturation rat”
• Chemistry in living systems (2005, background): “Furthermore, a collection of GFP-like tags is now available with emission wavelengths that span virtually the entire visible spectru”
• Photoactivatable mCherry for high-resolution two-color fluorescence microscopy (2009, neutral): “Here we report several irreversibly photoactivatable derivatives of mCherr”
• A High Precision Survey of the Molecular Dynamics of Mammalian Clathrin-Mediated Endocytosis (2011, methodology): “The amplified cDNAs were cloned into mammalian expression vectors in frame with a RFP (in the case of Hip1R, tDimer [65]; in the case of myosin1E, mApple [77]; and for all other proteins mCherry [78]; see Table S2) to generate either N- or C-terminal fusion proteins upon expression.”
• Guidelines for the use and interpretation of assays for monitoring autophagy (2012, background): “On the one hand, a putative advantage of mCherry over mRFP is its enhanced photostability and intensity, which are an order of magnitude higher (and comparable to GFP), enabling acquisition of images at similar exposure settings as are used for GFP, thus minimizing potential bias in interpretation.(”