Two-photon absorption properties of fluorescent proteins
By M. Drobizhev, N. Makarov, S. Tillo, T. Hughes, A. Rebane. Nature Methods, 2011
632 citations recorded by the source corpus.
Two-photon excitation of fluorescent proteins is an attractive approach for imaging living systems. Today researchers are eager to know which proteins are the brightest and what the best excitation wavelengths are. Here we review the two-photon absorption properties of a wide variety of fluorescent proteins, including new far-red variants, to produce a comprehensive guide to choosing the right fluorescent protein and excitation wavelength for two-photon applications.
Subfields: Biomedical Engineering, Biophysics, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/ba254093f690ccf5c73183718b0ea060557aff5f
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Citations in the corpus
- Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics (2013, background): “Red FPs frequently exhibit poor photostability (Drobizhev et al., 2011); this is a serious drawback that limits their utility in experiments.”
- Optimization of a GCaMP calcium indicator for neural activity imaging (2012, methodology): “Together with each run of GCaMP samples, a reference two-photon excitation spectrum of fluorescein was recorded, allowing us to determine the absolute two-photon cross section of the GCaMPs using published cross sections measured for these fluorophores (Xu and Webb, 1996; Drobizhev et al., 2011).”
- A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters (2018, neutral): “7, we present the two-photon action spectrum ( σ 2 φ ) for miRFP and compare it to that of EGFP (measured before 45 ).”