Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live Cells

By D. Zacharias, Jonathan D. Violin, A. Newton, Roger Tsien. Science, 2002

2,319 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Cell Biology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/3c58ffb97964246edf10758754d497e281acb2de

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Citations in the corpus

Engineering and characterization of a superfolder green fluorescent protein (2006, background): “A206K substantially reduces the tendency of GFP to dimerize even in concentrated solution”
Multi-step pericellular proteolysis controls the transition from individual to collective cancer cell invasion (2007, background): “Dashed lines mark the tip (1) and the base (2) of the leading pseudopod, pseudopod branching ( 3 ) and the final width of the cell body (4).”
Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae (2004, methodology): “…generated true monomeric CFP and YFP constructs which incorporate the Copyright 2004 John Wiley & Sons, Ltd. A206R mutation to block dimerization (Zacharias et al., 2002), and CFP and YFP constructs fused to the 3HA and 13Myc epitope tags (Evan et al., 1985; Field et al., 1988; Longtine et al.,…”
Creating new fluorescent probes for cell biology (2002, background): “The only instance when the weak tendency of AFPs to dimerize has been documented to cause artefacts or dysfunction was in the clustering of lipid-anchored AFPs on the plasma membran”
A Guide to Fluorescent Protein FRET Pairs (2016, background): “EGFP derivatives have been successfully monomerized with the mutations F223R, L221K, and A206K on the dimerization interface [109], all of which are outer barrel mutations that replace non-polar amino acids with hydrophilic alternatives.”
Direct visualization of Ras proteins in spatially distinct cell surface microdomains (2003, background): “A recent study has shown GFP dimerization compromises FRET analysis of protein–protein interactions in intact cells and results in lipid raft–targeted CFP and YFP continuing to display FRET when cells are treated with cyclodextrin (Zacharias et al., 2002).”
Aurora B-mediated abscission checkpoint protects against tetraploidization. (2009, neutral): “, 2006]), and plasma membrane (MyrPalm-mEGFP [Zacharias et al., 2002]), we found that cytokinetic furrow ingression always completed within 20 min after anaphase onset (n = 774), both in cells without chromosome bridges (n = 728), as well as in all cells with chromosome bridges (n = 46).”