Engineering and characterization of a superfolder green fluorescent protein

By J. Pedelacq, S. Cabantous, T. Tran, T. C. Terwilliger, G. Waldo. Nature Biotechnology, 2006

2,266 citations recorded by the source corpus.

Subfields: Biophysics, Molecular Biology, Radiology, Nuclear Medicine and Imaging

View Paper: https://www.semanticscholar.org/paper/f3235e830af8578619c2bc955955f7147bb3071a

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In Radiology, Nuclear Medicine and Imaging

Citations in the corpus

Toward machine-guided design of proteins (2018, background): “Break-Fix (sfGFP) - sfGFP is a variant of avGFP differing by 14 mutations that are the result of several directed evolution projects to improve avGFP’s brightness and stability [17].”
Removing T-cell epitopes with computational protein design (2014, methodology): “Proc Natl Acad Sci USA 108(17):6981–6986.”
Optimization of a GCaMP calcium indicator for neural activity imaging (2012, methodology): ““GCaMP-HS” consists of GCaMP2 with a subset of the “superfolder GFP” mutations (Pédelacq et al., 2006) and was used for imaging zebrafish motor neurons (Muto et al., 2011), but was also not compared with other sensors.”
Improving FRET dynamic range with bright green and red fluorescent proteins (2012, positive): “To test this hypothesis, we introduced into wild-type GFP the S65G mutation to maintain a deprotonated chromophore 28 and M153T and superfolder mutations to promote folding 29 .”
Design of a hyperstable 60-subunit protein icosahedron (2016, positive): “To probe the robustness of I3-01 to genetic fusion, we fused superfolder GFP (sfGFP) 26 to one or both termini of the monomeric subunit and produced the resulting proteins in E. coli.”
Type VI secretion requires a dynamic contractile phage tail-like structure (2012, positive): “Fluorescence microscopic imaging of the T6SS To test the hypothesis that the T6SS apparatus is a dynamic structure, we constructed a carboxy (C)-terminal fusion of VipA protein with superfolder green fluorescent protein (sfGFP”
Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. (2013, background): “The paused RNAP aborts transcription elongation upon encountering the dCas9-sgRNA roadblock. sfGFP (Pédelacq et al., 2006).”
Handbook of ion channels (2015, background): “In mammalian cells, incubated at 37°C, the maturation rate of FPs is relatively low and it would be prudent to use a version of superfolder GFP, which is optimized for expression at higher temperatures (122).”
GFP Reconstitution Across Synaptic Partners (GRASP) defines cell contacts and synapses in living nervous systems. (2008, background): “Moreover, superfolder GFP crystallizes as a monomer, suggesting that it should not serve as a nucleation site for further protein aggregation (Pedelacq et al., 2006).”
Low-N protein engineering with data-efficient deep learning (2020, background): “Additionally, they often require expert judgment to learn from data, which may include modifying energy functions for biophysical design 61 , and iteratively designing and testing structure-guided mutation combinations 19,62–65 .”