Peripheral CD103+ dendritic cells form a unified subset developmentally related to CD8α+ conventional dendritic cells

By B. Edelson, Wumesh Kc, Richard Juang, M. Kohyama, L. Benoît, P. Klekotka, Clara Moon, J. Albring, W. Ise, Drew G. Michael and 7 more. Journal of Experimental Medicine, 2010

744 citations recorded by the source corpus.

Although CD103-expressing dendritic cells (DCs) are widely present in nonlymphoid tissues, the transcription factors controlling their development and their relationship to other DC subsets remain unclear. Mice lacking the transcription factor Batf3 have a defect in the development of CD8α+ conventional DCs (cDCs) within lymphoid tissues. We demonstrate that Batf3−/− mice also lack CD103+CD11b− DCs in the lung, intestine, mesenteric lymph nodes (MLNs), dermis, and skin-draining lymph nodes. Notably, Batf3−/− mice displayed reduced priming of CD8 T cells after pulmonary Sendai virus infection, with increased pulmonary inflammation. In the MLNs and intestine, Batf3 deficiency resulted in the specific lack of CD103+CD11b− DCs, with the population of CD103+CD11b+ DCs remaining intact. Batf3−/− mice showed no evidence of spontaneous gastrointestinal inflammation and had a normal contact hypersensitivity (CHS) response, despite previous suggestions that CD103+ DCs were required for immune homeostasis in the gut and CHS. The relationship between CD8α+ cDCs and nonlymphoid CD103+ DCs implied by their shared dependence on Batf3 was further supported by similar patterns of gene expression and their shared developmental dependence on the transcription factor Irf8. These data provide evidence for a developmental relationship between lymphoid organ–resident CD8α+ cDCs and nonlymphoid CD103+ DCs.

Subfields: Immunology

View Paper: https://www.semanticscholar.org/paper/3bec23c6063f042f0ffe6d5e111a8705d9293033

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Immunology

1. Steffen Jung
2. Florent Ginhoux
3. Robert Schreiber
4. C. Varol
5. Miriam Merad
6. T. Gajewski
7. C. Arthur
8. G. Dunn

Citations in the corpus

• Human Tissues Contain CD141hi Cross-Presenting Dendritic Cells with Functional Homology to Mouse CD103+ Nonlymphoid Dendritic Cells (2012, background): “development suggest that both are dependent upon the function of IRF8, Id2, and Batf3 (Aliberti et al., 2003; Edelson et al., 2010) and both arise from a common precursor, the pre-DC (Ginhoux et al.”
• Type I interferon is selectively required by dendritic cells for immune rejection of tumors (2011, positive): “…were originally identified as the CD8  + DCs that resided in lymphoid organs; yet subsequent work showed that they are closely related to another small DC subset residing in peripheral tissues that lack CD8  but express CD103 (Hildner et al., 2008; Ginhoux et al., 2009; Edelson et al., 2010).”
• Critical Role for CD103+/CD141+ Dendritic Cells Bearing CCR7 for Tumor Antigen Trafficking and Priming of T Cell Immunity in Melanoma (2016, positive): “Our work is consistent with theBatf3 result insofar aswe foundCD103+ DC to be essential, and these rely upon BATF3 (Edelson et al., 2010).”
• The dendritic cell lineage: ontogeny and function of dendritic cells and their subsets in the steady state and the inflamed setting. (2013, background): “These results are consistent with the finding that constitutive deletion of CD11c+ DCs leads to autoimmune disorders (212), although autoimmune reactions were not reported in another strain of mice that constitutively lacks CD11c+ cells (97) or in Batf3−/− mice that lack CD8+ and CD103+ DCs (132, 14”
• Ly6C hi monocytes in the inflamed colon give rise to proinflammatory effector cells and migratory antigen-presenting cells. (2012, background): “Like in lymphoid organs, these classical DCs can be further subdivided according to their dependence on the transcription factor BatF3 into CD11b! and CD11b subpopulations (Edelson et al., 2010).”
• Characterization of human DNGR-1+ BDCA3+ leukocytes as putative equivalents of mouse CD8α+ dendritic cells (2010, background): “However, Batf3-dependent CD11b– CD103+ Langerin+ DCs related to CD8+ DCs have recently been identified in mouse peripheral tissues (Bedoui et al., 2009; Ginhoux et al., 2009; Edelson et al., 2010; Henri et al., 2010).”
• Tumor-residing Batf3 dendritic cells are required for effector T cell trafficking and adoptive T cell therapy (2017, methodology): “To investigate a direct role for Batf3-lineage CD103+ DCs in effector T cell recruitment, we generated CD11c-DTR/Batf3 / or CD11c-DTR/WT mixed bone marrow chimeras into BP-SIY host mice, as Batf3 is known to be required for development of CD103+ DCs (Broz et al., 2014; Edelson et al., 2010).”