Microbial stimulation fully differentiates monocytes to DC-SIGN/CD209+ dendritic cells for immune T cell areas

By C. Cheong, I. Matos, Jae-Hoon Choi, D. Dandamudi, Elina Shrestha, M. Longhi, Kate L. Jeffrey, Robert M. Anthony, Courtney Kluger, G. Nchinda and 7 more. Cell, 2010

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SUMMARY Dendritic cells (DCs), critical antigen presenting cells for immune control, normally derive from bone marrow precursors distinct from monocytes. It is not yet established if the large reservoir of monocytes can develop into cells with critical features of DCs in vivo. We now show that fully differentiated Mo-DCs develop in mice and DC-SIGN/CD209a marks the cells. Mo-DCs are recruited from blood monocytes into lymph nodes by lipopolysaccharide and live or dead gram negative bacteria. Mobilization requires TLR4 and its CD14 coreceptor and Trif. When tested for antigen presenting function, Mo-DCs are as active as classical DCs, including cross presentation of proteins and live gram negative bacteria on MHC I in vivo. Fully differentiated Mo-DCs acquire DC morphology and localize to T cell areas via L-selectin and CCR7. Thus the blood monocyte reservoir becomes the dominant presenting cell in response to select microbes, yielding DC-SIGN+ cells with critical functions of DCs.

Subfields: Immunology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/3a3b0690cfd435e881e2b36141abace6635ca3a0

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Citations in the corpus

Zbtb46 expression distinguishes classical dendritic cells and their committed progenitors from other immune lineages (2012, background): “8 B). similarity to GM-CSF–derived DCs (Cheong et al., 2010).”
CD14 controls the LPS-induced endocytosis of Toll-like Receptor 4 (2011, background): “CD14 has recently been implicated in regulating the differentiation of monocyte-derived DCs (Mo-DCs) upon encountering LPS (Cheong et al., 2010).”
Neutrophils in the activation and regulation of innate and adaptive immunity (2011, background): “It has recently been demonstrated that, in mice treated with LPS or Gram-negative bacteria, peripheral monocytes migrate to lymph nodes, where they differentiate into DCs to become the predominant antigen-presenting cell”
Transcriptional regulation of macrophage polarization: enabling diversity with identity (2011, background): “Recent research has also highlighted the important contribution of monocyte-derived antigen-presenting cells in priming T cell responses and adaptive immunit”
The spleen in local and systemic regulation of immunity (2013, background): “…cells) or patrol blood vessels (CX3CR1hiLy6Clo cells) (Geissmann et al., 2010) but irreversibly differentiate intoDCsormacrophages upon recruitment to either lymphoid (Serbina et al., 2003; Cheong et al., 2010) or nonlymphoid tissues (Nahrendorf et al., 2007; Grainger et al., 2013).”