A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum
By Nathan Christopher Shaner, G. G. Lambert, Andrew Chammas, Yuhui Ni, Paula J. Cranfill, M. A. Baird, Brittney R. Sell, John R. Allen, R. Day, M. Israelsson and 2 more. Nature Methods, 2013
1,233 citations recorded by the source corpus.
We report a monomeric yellow-green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum. mNeonGreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single-molecule superresolution imaging and is an excellent fluorescence resonance energy transfer (FRET) acceptor for the newest cyan fluorescent proteins.
Subfields: Biophysics, Molecular Biology, Pharmacology
View Paper: https://www.semanticscholar.org/paper/fd48e7bb562b0ba699499c0d547b3d0dec6c287f
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Biophysics
- Michael Z. Lin
- Roger Tsien
- Robert E. Campbell
- Sergey A. Lukyanov
- Konstantin A. Lukyanov
- Dmitriy M. Chudakov
In Molecular Biology
- Robert E. Campbell
- Michael Z. Lin
- Roger Tsien
- A. Palmer
- Sergey A. Lukyanov
- Konstantin A. Lukyanov
- Vasilis Ntziachristos
- Dmitriy M. Chudakov
Citations in the corpus
- The growing and glowing toolbox of fluorescent and photoactive proteins (2016, background): “In contrast, mNeonGreen emerged from an underexplored lineage and was created by incorporating 21 mutations into the yellow FP derived from Branchiostoma lanceolatum, LanYFP [17], suggesting that there remains untapped potential in additional marine species.”
- A Guide to Fluorescent Protein FRET Pairs (2016, background): “Bright green FP donors include Clover and mClover3, derived from GFP, and mNeonGreen, derived from Branchiostoma lanceolatum YFP [45,46].”
- OpenCell: proteome-scale endogenous tagging enables the cartography of human cellular organization (2022, neutral): “Finally,relyingonendogenousexpression canbeanobstaclegiventhelowconcentration ofmostproteinsinthehumancell:Detection ofpoorlyabundantproteinsisdifficult(espe-ciallythoseinthebottomhalfoftheabundance distribution)evenwhenusingaverybrightFP suchasmNeonGreen( 74 ) (fig.”