Metabolism and regulation of canonical histone mRNAs: life without a poly(A) tail

By W. Marzluff, Eric J. Wagner, R. Duronio. Nature reviews genetics, 2008

762 citations recorded by the source corpus.

The canonical histone proteins are encoded by replication-dependent genes and must rapidly reach high levels of expression during S phase. In metazoans the genes that encode these proteins produce mRNAs that, instead of being polyadenylated, contain a unique 3′ end structure. By contrast, the synthesis of the variant, replication-independent histones, which are encoded by polyadenylated mRNAs, persists outside of S phase. Accurate positioning of both histone types in chromatin is essential for proper transcriptional regulation, the demarcation of heterochromatic boundaries and the epigenetic inheritance of gene expression patterns. Recent results suggest that the coordinated synthesis of replication-dependent and variant histone mRNAs is achieved by signals that affect formation of the 3′ end of the replication-dependent histone mRNAs.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/3446fca7422b51548e5b243a9e1de79baa9d68dc

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Citations in the corpus

• Epigenetics in alternative pre-mRNA splicing (2011, neutral): “The reason appears to be failure to recruit histone RNA-processing factors to nascent histone RNAs (Marzluff et al., 2008).”
• The Nucleolus under Stress (2010, neutral): “In S phase, the recruitment of the cyclinE/cdk2 complex to CBs leads to the phosphorylation of NPAT and the subsequent activation of histone mRNA transcription (Marzluff et al., 2008) .”
• Regulation of cytoplasmic mRNA decay (2012, neutral): “Cell-cycle-regulated histone mRNAs are exceptional because they lack a poly(A) tail and instead end in a conserved stem–loop structure that binds stem–loop-binding protein (SLBP), which is only present during S phase and is crucial for efficient histone mRNA 3′ end formation in the nucleus and for e”
• Chromatin replication and epigenome maintenance (2012, background): “2, H4, H2A, H2B and H1) in S phase is tightly regulated at the level of transcription, translation and mRNA stabilit”
• mRNA-Seq whole-transcriptome analysis of a single cell (2009, methodology): “First, because the single-cell cDNA amplification method relies on poly(T) primer, which can only capture mRNA with a poly(A) tail, mRNAs without poly(A) tails, such as histone mRNAs, will not be detected by our mRNA-Seq assa”
• Ending the message: poly(A) signals then and now. (2011, background): “This RNA, in association with multiple protein components (forming U7snRNP), is recruited to histone mRNA 39 ends by interaction with additional factors, including a zinc finger protein and hairpin-binding protein (Marzluff et al. 2008).”