The ESCRT pathway.

By W. Henne, Nicholas J. Buchkovich, S. Emr. Developmental Cell, 2011

1,453 citations recorded by the source corpus.

Multivesicular bodies (MVBs) deliver cargo destined for degradation to the vacuole or lysosome. The ESCRT (endosomal sorting complex required for transport) pathway is a key mediator of MVB biogenesis, but it also plays critical roles in retroviral budding and cytokinetic abscission. Despite these diverse roles, the ESCRT pathway can be simply seen as a cargo-recognition and membrane-sculpting machine viewable from three distinct perspectives: (1) the ESCRT proteins themselves, (2) the cargo they sort, and (3) the membrane they deform. Here, we review ESCRT function from these perspectives and discuss how ESCRTs may drive vesicle budding.

Subfields: Cell Biology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/33b261f45110168c733b643f507a8ae0eeb30877

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Citations in the corpus

Formation and release of arrestin domain-containing protein 1-mediated microvesicles (ARMMs) at plasma membrane by recruitment of TSG101 protein (2012, background): “Using a unique ubiquitin enzyme variant (UEV) domain that binds to ubiquitin and recognizes the tetrapeptide protein motif PS/TAP (7–9), TSG101 interacts with the early endosomal protein HRS (6, 10, 11) and the late endosome-associated protein ALIX (12) to mediate the trafficking of ubiquitinated re”
SORTING IT OUT: REGULATION OF EXOSOME LOADING (2014, background): “Ubiquitination is involved in the ESCRT-complex-mediated sorting of proteins into ILVs for their later degradation [20]; however, its involvement in the sorting of cargo into exosomes is not so clear.”
Extracellular Vesicle Heterogeneity: Subpopulations, Isolation Techniques, and Diverse Functions in Cancer Progression (2018, background): “Exosome biogenesis depends on the endosomal sorting complex required for transport (ESCRT) machinery that is responsible for protein sorting and ILV formation (50, 51).”
Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles. (2014, background): “Of note, MVB-independent functions of the ESCRT machinery, such as budding of PM-derived vesicles, membrane fission during cytokinesis (Henne et al. 2011), or plasma membrane repair ( Jimenez et al. 2014), do not involve the ESCRT-0 proteins HRS and STAM.”
The delivery of therapeutic oligonucleotides (2016, background): “Thus the membrane lipid and protein composition of exosomes closely parallels those of the LE/MVB compartment.”
Introduction to Extracellular Vesicles: Biogenesis, RNA Cargo Selection, Content, Release, and Uptake (2016, neutral): “In turn, ESCRT III is recruited through programmed cell death 6 interacting protein (PDCD6IP or ALIX), and associates with the tumor susceptibility gene 101 (TSG101) as part of the ESCRT I complex (Matsuo et al. 2004; Baietti et al. 2012).”
Analysis of ESCRT functions in exosome biogenesis, composition and secretion highlights the heterogeneity of extracellular vesicles (2013, background): “The discovery that the ESCRT machinery is involved in MVB formation (Henne et al., 2011; Raiborg and Stenmark, 2009) opened a new avenue to interfere with the formation of ILVs and thereby exosome secretion.”