Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin.

By A. Bolotin, Benoit Quinquis, A. Sorokin, S. Ehrlich. Microbiology, 2005

940 citations recorded by the source corpus.

Subfields: Ecology, Epidemiology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/3393a40ec965378e784a0f0fe436f813db22687a

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology

1. Feng Zhang
2. Eugene V. Koonin
3. Rodolphe Barrangou
4. L. Marraffini
5. Bernd Zetsche
6. Phillip A. Sharp
7. Jennifer A. Doudna
8. Kira S. Makarova

In Epidemiology

1. Feng Zhang
2. Rodolphe Barrangou
3. Le Cong
4. Jennifer A. Doudna

In Molecular Biology

1. Feng Zhang
2. Jennifer A. Doudna
3. Eugene V. Koonin
4. Rodolphe Barrangou
5. Bernd Zetsche
6. Jonathan Gootenberg
7. L. Marraffini
8. David A. Scott

Citations in the corpus

• In vivo genome editing using Staphylococcus aureus Cas9 (2015, background): “Cas9, an RNA-guided endonuclease derived from the Type II CRISPR-Cas bacterial adaptive immune system 1–7 , has been harnessed for genome editing 8,9 and holds tremendous promise for biomedical research.”
• Biology and Applications of CRISPR Systems: Harnessing Nature's Toolbox for Genome Engineering. (2016, neutral): “Nearly two decades after CRISPR loci were first identified in Escherichia coli, spacers were found to derive from viral genomes and conjugative plasmids, serving as records of previous infection (Bolotin et al., 2005; Ishino et al., 1987; Mojica et al., 2005; Pourcel et al., 2005).”
• Point-of-care CRISPR/Cas nucleic acid detection: Recent advances, challenges and opportunities (2020, positive): “It took many years to show that CRISPR and Cas genes were part of the adaptive immune system of bacteria, used as a defense mechanism against foreign nucleotides (Bolotin et al., 2005; Mojica et al., 2005; Pourcel et al., 2005).”
• The Biology of CRISPR-Cas: Backward and Forward. (2018, neutral): “A milestone in CRISPR research was the realization that the spacer sequences match plasmids and phage genomes, as it was the first hint that CRISPR-Cas might function as a prokaryotic defense mechanism (Bolotin et al., 2005; Mojica et al., 2005; Pourcel et al., 2005).”
• CRISPR-Cas systems: prokaryotes upgrade to adaptive immunity (2014, background): “Their biological role remained elusive until 2005, when three groups reported that the spacers were homologous to foreign genetic elements, including viruses and plasmids (Bolotin et al., 2005; Mojica et al., 2005; Pourcel et al., 2005).”