An inexpensive, automation-friendly protocol for recovering high-quality DNA

By Natalia N. Ivanova, Jeremy R. deWaard, Paul D. N. Hebert. 2006

1,486 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/2edb54a08875e24c45179f34a8b3140e76ed77ad

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In Molecular Biology

Citations in the corpus

Integrative inventory of Bolivia’s lowland anurans reveals hidden diversity (2011, methodology): “DNA was extracted from muscle tissue using a standard glass fibre extraction protocol (Ivanova et al. 2006).”
Prospects for using DNA barcoding to identify spiders in species-rich genera (2009, methodology): “A standard Glass-Fibre protocol, (Ivanova et al. 2006) was used for DNA extraction.”
Can DNA barcodes of stream macroinvertebrates improve descriptions of community structure and water quality? (2011, methodology): “Genomic mitochondrial DNA was extracted, and the 657 base pair (bp) barcoding region of the cytochrome c oxidase subunit 1 (COI) gene was amplified and sequenced using standard barcoding protocols (Ivanova et al. 2006) at the University of Guelph Canadian Centre for DNA Barcoding.”
Now DNA-barcoded: the butterflies and larger moths of Germany (Lepidoptera: Rhopalocera, Macroheterocera) (2011, methodology): “PCR amplification and DNA sequencing was performed at the CCDB following standard high-throughput protocols (Ivanova et al. 2006, deWaard et al. 2008), that can be accessed under http://www.dnabarcoding.ca/pa/ ge/research/protocols.”
DNA barcoding detects market substitution in North American seafood (2008, methodology): “DNA barcoding now takes advantage of streamlined and inexpensive protocols ( Ivanova, DeWaard, & Hebert, 2006; Ivanova, Zemlak, Hanner, & Hebert, 2007) that facilitate processing hundreds of thousands of samples per year within a single DNA barcoding core facility (Hajibabaei et al., 2005).”
Ephemeroptera, Plecoptera, and Trichoptera fauna of Churchill (Manitoba, Canada): insights into biodiversity patterns from DNA barcoding (2010, methodology): “Standard barcoding protocols for DNA extraction (Ivanova et al. 2006), polymerase chain reaction (PCR) amplification, and sequencing (deWaard et al. 2008, Hajibabaei et al. 2005) were followed.”
DNA barcoding of Neotropical bats: species identification and discovery within Guyana (2007, methodology): “Samples producing single clear amplicons from cocktail 1 were sequenced with VF1d and VR1d (Ivanova et al. 2006), while those from cocktail 2 were sequenced with M13F and M13R (Messing 1983) using BigDye version 3.1 on an ABI PRISM 3730 capillary sequencer (Applied Biosystems).”
Challenging species delimitation in Collembola: cryptic diversity among common springtails unveiled by DNA barcoding (2012, methodology): “DNA extraction followed a standard automated protocol using 96-well glass fibre plates (Ivanova et al. 2006).”
The campaign to DNA barcode all fishes, FISH-BOL. (2009, background): “Barcoding typically uses inexpensive high-throughput extraction (Ivanova et al., 2006), and sequencing (Hajibabaei et al., 2005) protocols coupled with primers and primer cocktails developed for fishes (Ward et al., 2005; Ivanova et al., 2007).”
Semi-automated, Membrane-Based Protocol for DNA Isolation from Plants (2008, methodology): “tissues (Ivanova et al. 2006) we employed binding of DNA to glass fiber in the presence of guanidine thiocyanate (GuSCN) and ethanol.”