Applications of next-generation sequencing technologies in functional genomics.

By Olena Morozova, Marco A. Marra. Genomics, 2008

1,237 citations recorded by the source corpus.

A new generation of sequencing technologies, from Illumina/Solexa, ABI/SOLiD, 454/Roche, and Helicos, has provided unprecedented opportunities for high-throughput functional genomic research. To date, these technologies have been applied in a variety of contexts, including whole-genome sequencing, targeted resequencing, discovery of transcription factor binding sites, and noncoding RNA expression profiling. This review discusses applications of next-generation sequencing technologies in functional genomics research and highlights the transforming potential these technologies offer.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/d39794fcf2a372dbb507606e48ccb70ef23d58c1

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Citations in the corpus

De novo assembly and characterization of the spleen transcriptome of common carp (Cyprinus carpio) using Illumina paired-end sequencing. (2015, background): “Recently, rapid developments in high-throughput deep sequencing technologies have provided a powerful platform for the rapid and nearly complete characterization of transcriptomic events in various species [14,15].”
Nucleic acid-based approaches to investigate microbial-related cheese quality defects (2012, methodology): “Therefore, each cluster is sequenced by the color associated with the nucleotide added (Mardis, 2008; Morozova and Marra, 2008; Zhang et al., 2011).”
Design of a High Density SNP Genotyping Assay in the Pig Using SNPs Identified and Characterized by Next Generation Sequencing Technology (2009, methodology): “In recent years, new sequencing technologies have emerged which offer great promise for marker discovery due to their ability to efficiently generate large amounts of sequence data, both in terms of time and cost. They are usually referred to as “second generation” or “next generation” sequencing te”
ngs.plot: Quick mining and visualization of next-generation sequencing data by integrating genomic databases (2014, background): “Its ability to produce more than one billion sequencing reads within the timeframe of a few days [1] has enabled the investigation of tens of thousands of biological events in parallel [2,3].”
Applications of next generation sequencing in molecular ecology of non-model organisms (2011, neutral): “We will not go into any technical details of NGS because this has already been extensively reviewed elsewhere (Hudson, 2008; Morozova and Marra, 2008; Shendure and Ji, 2008).”
Economic Importance, Breeding Objectives and Achievements (2011, methodology): “These include the Illumina/ Solexa, ABI/SOLiD, 454/Roche, Pacifi c Biosciences/SMRT and Helicos (Morozova and Marra 2008).”
A novel class of small RNAs: tRNA-derived RNA fragments (tRFs). (2009, background): “We believe that additional small RNAs await discovery, especially through the application of high-throughput sequencing technology (for review, see Morozova and Marra 2008).”
A Parsimony Approach to Biological Pathway Reconstruction/Inference for Genomes and Metagenomes (2009, neutral): “sequencing technologies [1].”