Assessment of the sensitivity and specificity of oligonucleotide (50mer) microarrays.
By M. Kane, T. Jatkoe, C. Stumpf, Jiasen Lu, J. D. Thomas, S. Madore. Nucleic Acids Research, 2000
680 citations recorded by the source corpus.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/2d48a1aa0ff90ec29655dc557ca4b6540c9deb28
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Citations in the corpus
- Navigating gene expression using microarrays — a technology review (2001, background): “Although short oligonucleotides may result in less specific hybridization and reduced sensitivity, the arraying of presynthesized longer oligonucleotides (50–100mers) has recently been developed to counteract these disadvantage”
- Direct multiplexed measurement of gene expression with color-coded probe pairs (2008, methodology): “The cross-hybridization cutoffs were chosen based on prior 50-base hybridization and probe design studie”
- Correlation of mRNA and protein in complex biological samples (2009, neutral): “Similar results have been found for other systems and platforms [73,74].”
- Physiology and molecular biology of stress tolerance in plants (2006, background): “For increasing the specificity, 50-mer and 70-mer probe arrays have also been designed and it was found that there were no difference between these arrays and full length cDNA arrays (Kane et al., 2000; Ten-Bosch et al., 2001).”
- Reliability and reproducibility issues in DNA microarray measurements. (2006, background): “Finally, Kane et al. compared the sensitivity of 50mer oligonucleotide probes with that of PCR products [ 17 ].”
- OligoArray 2.0: design of oligonucleotide probes for DNA microarrays using a thermodynamic approach. (2003, background): “These authors and others (7) have also studied the speci®city limit of short oligonucleotides in terms of sequence identity with mRNAs other than their target and have shown greater than 66% identity can lead to crosshybridization.”