Correlation of mRNA and protein in complex biological samples
By Tobias Maier, Marc Güell, Luis Serrano. FEBS Letters, 2009
1,789 citations recorded by the source corpus.
The correlation between mRNA and protein abundances in the cell has been reported to be notoriously poor. Recent technological advances in the quantitative analysis of mRNA and protein species in complex samples allow the detailed analysis of this pathway at the center of biological systems. We give an overview of available methods for the identification and quantification of free and ribosome‐bound mRNA, protein abundances and individual protein turnover rates. We review available literature on the correlation of mRNA and protein abundances and discuss biological and technical parameters influencing the correlation of these central biological molecules.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/9a1d80ecfda8c0a2fcf1738296f4f5e4225d5040
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In Molecular Biology
- Jüergen Cox
- Matthias Mann
- T. Geiger
- Jacek R. Wisniewski
- Nagarjuna Nagaraj
- Martin Kircher
- Janet Kelso
- Svante Pääbo
Citations in the corpus
- Deep proteome and transcriptome mapping of a human cancer cell line (2011, background): “…higher correlations between transcriptomes and proteomes are observed here than in previous studies (Cox and Mann, 2011; de Sousa Abreu et al, 2009; Maier et al, 2009), where technical imperfections in the quantification of both the proteome and the transcriptome are likely to have reduced their…”
- Insights into the regulation of protein abundance from proteomic and transcriptomic analyses (2012, neutral): “From the above technologies, we now have measurements of the absolute concentrations of mRNAs and proteins from various organisms, including mammalian cells, worms, flies, yeast and a few species of bacteri”