Reliability and reproducibility issues in DNA microarray measurements.

By Sorin Draghici, Purvesh Khatri, A. Eklund, Zoltan Szallasi. Trends in Genetics, 2006

626 citations recorded by the source corpus.

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the current technology has several limitations. Here we discuss problems related to the sensitivity, accuracy, specificity and reproducibility of microarray results. The existing data suggest that for relatively abundant transcripts the existence and direction (but not the magnitude) of expression changes can be reliably detected. However, accurate measurements of absolute expression levels and the reliable detection of low abundance genes are difficult to achieve. The main problems seem to be the sub-optimal design or choice of probes and some incorrect probe annotations. Well-designed data-analysis approaches can rectify some of these problems.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/792ab437ecafd8c5b4b5f77eeb6082917b97e1e9

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In Molecular Biology

Citations in the corpus

Advances and Trends in Omics Technology Development (2022, background): “Besides, DNA microarray is not a feasible platform when analyzing highly repetitive genomes due to the high occurrence of cross-hybridization events that may lead to inaccurate signal intensity estimation (61).”
PrognoScan: a new database for meta-analysis of the prognostic value of genes (2009, background): “In addition, it is known that a substantial number of incorrect probes are used in microarrays [60].”
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Correlation of mRNA and protein in complex biological samples (2009, neutral): “They can be used to measure absolute transcript concentrations whenever the probe binds to its target speciﬁcally [3].”
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