Automated molecular microscopy: the new Leginon system.

By C. Suloway, Jim Pulokas, D. Fellmann, Anchi Cheng, Francisco Guerra, J. Quispe, S. Stagg, Clinton S. Potter, Bridget O. Carragher. 2005

1,799 citations recorded by the source corpus.

Subfields: Atomic and Molecular Physics, and Optics, Biophysics, Structural Biology, Computational Biology & Bioinformatics

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Atomic and Molecular Physics, and Optics

In Biophysics

In Structural Biology

In Computational Biology & Bioinformatics

Citations in the corpus

An Integrated Micro- and Macroarchitectural Analysis of the Drosophila Brain by Computer-Assisted Serial Section Electron Microscopy (2010, methodology): “For image capturing, we used the software Leginon (Automated Molecular Imaging group at the Scripps Institute, San Diego, CA).”
Induction of Potent Neutralizing Antibody Responses by a Designed Protein Nanoparticle Vaccine for Respiratory Syncytial Virus (2019, methodology): “Data was collected using the Leginon software (Suloway et al., 2005) on an FEI TF20 electron microscope, equipped with a Gatan K2 Summit direct electron detector.”
RELION: Implementation of a Bayesian approach to cryo-EM structure determination (2012, methodology): “More convenient data collection schemes are being developed through a combination of automated data acquisition software (Suloway et al., 2005) and improvements in the”
Leginon: New features and applications (2020, methodology): “The current Leginon framework was published in 2005.(3) Updates to support new microscopes, cameras and peripheral services are included in version releases on an ongoing basis.”
Crowdsourcing the creation of image segmentation algorithms for connectomics (2015, methodology): “The images were captured using Leginon (Suloway et al., 2005) to drive a FEI electron microscope equipped with a Tietz camera and a goniometer-powered mobile grid stage, with a magniﬁcation of 5600 × binned at 2, which delivers the 4 × 4 nm per pixel resolution.”
Antibody Recognition of the Pandemic H1N1 Influenza Virus Hemagglutinin Receptor Binding Site (2013, methodology): “Leginon software package (19, 20) was used to automate some steps of”
Presenting native-like trimeric HIV-1 antigens with self-assembling nanoparticles (2016, neutral): “4k TemCam-F416 CMOS camera using a nominal defocus of 1,000nm and the Leginon package 66 .”
Generation of ordered protein assemblies using rigid three-body fusion (2020, neutral): “…(37 – 41), 3) SAXS sample preparation and analytical tools for data collected at the Structurally Integrated Biology for Life Sciences (SIBYLS) Beamline (42 – 44), 4) negative-stain EM procedures and tools (32, 33, 45, 46), and 5) cryo-EM sample preparation and processing schemes (32, 33, 46 – 57).”
Single-Particle Cryo-EM at Crystallographic Resolution. (2015, methodology): “…image phase error induced by beam tilt (Glaeser et al., 2011), very high vacuum to reduce water contamination on frozen-hydrated samples loaded into the microscope column, and better computer control for sophisticated and automated microscope tuning and data acquisition (Suloway et al., 2005), etc.”
Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein (2020, methodology): “Data were acquired using the Leginon software (Suloway et al., 2005) to control an FEI Titan Krios transmission electron microscope operated at 300 kV and equipped with a Gatan K2 Summit direct detector and Gatan Quantum GIF energy filter, operated in zero-loss mode with a slit width of 20 eV.”