Structure of the 70S Ribosome Complexed with mRNA and tRNA

By M. Selmer, C. Dunham, F. Murphy, A. Weixlbaumer, S. Petry, A. Kelley, J. Weir, V. Ramakrishnan. Science, 2006

1,338 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/265a267989d3618d76bed4f921073946dcd487c6

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Citations in the corpus

Initiation factor 2 stabilizes the ribosome in a semirotated conformation (2015, background): “subunit interface (Protein Data Bank ID code 4V51) (45).”
Studies on the role of helix 69 of 23S rRNA in the factor-dependent stages of translation initiation, elongation, and termination (2011, background): “The E-site is formed by helices H68, H74–77 and H88 of 23S rRNA plus protein L1 on the 50S side (Rinke-Appel et al. 1995; Yusupov et al. 2001).”
The spliceosome: design principles of a dynamic RNP machine. (2009, background): “Structural (Ogle et al., 2001; Selmer et al., 2006; Simonovic and Steitz, 2008) and kinetic studies (Wohlgemuth et al.”
Structural Changes Enable Start Codon Recognition by the Eukaryotic Translation Initiation Complex (2014, background): “In bacteria, recognition of the minor groove of the first two base pairs by G1338 and A1339 of 16S rRNA was suggested to stabilize the binding of tRNAi in the P site (Lancaster and Noller, 2005; Qin et al., 2007), as subsequently seen in 70S complexes (Selmer et al., 2006; Korostelev et al., 2006).”
Structural Insights into RNA Dimerization: Motifs, Interfaces and Functions (2020, background): “This strategy is reminiscent of the stabilization of codon-anticodon interactions in the ribosome [81,82] and T-box riboswitches [83–86], as well as a 3-bp pseudoknot in the adenovirus VA-I RNA [87].”
On the application of the expected log-likelihood gain to decision making in molecular replacement (2018, background): “…complex with the guanosine triphosphatase release factor 3 (PDB entry 3zvo) can be solved with the 30S (PDB entry 2j00) and 50S (PDB entry 2j01) components of the structure of the Thermus thermophilus 70S ribo- some complexed with mRNA, tRNA and paromomycin (Selmer et al., 2006; Jin et al., 2011).”
Regulation of Translation Initiation in Eukaryotes: Mechanisms and Biological Targets (2009, positive): “The stunning progress on crystal structures of bacterial 70S ribosomes bound to mRNAs and tRNAs (Selmer et al., 2006; Yusupova et al., 2006) has provided atomic details of the path of mRNA and contacts made with tRNAs in the decoding sites.”
RELION: Implementation of a Bayesian approach to cryo-EM structure determination (2012, methodology): “For this purpose, a set of atomic coordinates of the 70S ribosome (PDB-IDs 2J00 and 2J01) (Selmer et al., 2006)was converted to a densitymapof 128 128 128 voxels,with a voxel size of 2.”
The scanning mechanism of eukaryotic translation initiation. (2014, background): “In addition to base-pairing with AUG, the ASL interacts with multiple 18S rRNA helices that constitute the P site, similarly to P-site tRNA in bacterial 70S ribosomes (192, 193), except that the tRNAi is tilted toward the E site [reminiscent of the P/I state of bacterial 30S ICs (194)].”
The initiation of mammalian protein synthesis and the mechanism of scanning (2013, background): “This pocket is formed by the 18S rRNA helices h28, h44 of the body, h24 of the platform, h28, h29, h30, h31 of the head domains and by three ribosomal proteins (rpS) located in the head of 40S subunit: rpS15(19), rpS16(9) and rpS18(13) (Fig.”