Expression of Cd34 and Myf5 Defines the Majority of Quiescent Adult Skeletal Muscle Satellite Cells

By J. Beauchamp, L. Heslop, David S.W. Yu, S. Tajbakhsh, R. Kelly, A. Wernig, Margaret E. Buckingham, T. Partridge, P. Zammit. Journal of Cell Biology, 2000

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Skeletal muscle is one of a several adult post-mitotic tissues that retain the capacity to regenerate. This relies on a population of quiescent precursors, termed satellite cells. Here we describe two novel markers of quiescent satellite cells: CD34, an established marker of hematopoietic stem cells, and Myf5, the earliest marker of myogenic commitment. CD34+ve myoblasts can be detected in proliferating C2C12 cultures. In differentiating cultures, CD34+ve cells do not fuse into myotubes, nor express MyoD. Using isolated myofibers as a model of synchronous precursor cell activation, we show that quiescent satellite cells express CD34. An early feature of their activation is alternate splicing followed by complete transcriptional shutdown of CD34. This data implicates CD34 in the maintenance of satellite cell quiescence. In heterozygous Myf5 nlacZ/+ mice, all CD34+ve satellite cells also express β-galactosidase, a marker of activation of Myf5, showing that quiescent satellite cells are committed to myogenesis. All such cells are positive for the accepted satellite cell marker, M-cadherin. We also show that satellite cells can be identified on isolated myofibers of the myosin light chain 3F-nlacZ-2E mouse as those that do not express the transgene. The numbers of satellite cells detected in this way are significantly greater than those identified by the other three markers. We conclude that the expression of CD34, Myf5, and M-cadherin defines quiescent, committed precursors and speculate that the CD34−ve, Myf5−ve minority may be involved in maintaining the lineage-committed majority.

Subfields: Genetics, Molecular Biology, Physiology

View Paper: https://www.semanticscholar.org/paper/22cd8493985f51cb153dfae75faa86ccf350fa16

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Citations in the corpus

The formation of skeletal muscle: from somite to limb (2003, background): “They also express some endothelial cell markers (Beauchamp et al. 2000).”
Making muscle: skeletal myogenesis in vivo and in vitro (2017, background): “A majority of the adult Pax7 satellite cells also expressesMyf5, but these are distinct from proliferating Myf5 myoblasts which also express MyoD (Beauchamp et al., 2000; Cornelison and Wold, 1997; Zammit et al., 2004) (Fig.”
Cellular and molecular regulation of muscle regeneration. (2004, background): “Another interesting cell population is the Sca-1 / CD34 population isolated from murine muscle (151, 245, 317).”
Satellite cells and the muscle stem cell niche. (2013, background): “Examination of -galactosidase activity in Myf5-LacZ mice indicated that the Myf5 locus is active in 90% of quiescent satellite cells, which suggests most satellite cells are committed to the myogenic lineage (37).”
Muscle satellite cells adopt divergent fates (2004, methodology): “Immunostaining Fixed myofibers were permeabilized with 0.5% (vol/vol) Triton X-100/PBS and blocked using 20% (vol/vol) goat serum/PBS, as described previously (Beauchamp et al., 2000).”
Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells (2006, background): “Myogenic regulatory genes are expressed during this process; Myf5 is already expressed in quiescent satellite cells (Beauchamp et al., 2000), and MyoD is expressed as the cells become activated and subsequently differentiate with the expression of myogenin (Yablonka-Reuveni and Rivera, 1994).”
Myogenic satellite cells: physiology to molecular biology. (2001, background): “M-cadherin is only expressed in a subpopulation of the quiescent cell pool; however, its expression is increased when the satellite cells become activated in response to a stimulus (9, 33).”
Myogenic specification of side population cells in skeletal muscle (2002, methodology): “4% collagenase type A (Roche) as described previously (Beauchamp et al., 2000).”
Asymmetric self-renewal and commitment of satellite stem cells in muscle. (2007, background): “Examination of the expression of satellite cell markers CD34, M-cadherin, and Myf5-nLacZ in freshly prepared myofibers suggested that a subpopulation of satellite cells exhibits heterogeneous expression of these markers (Beauchamp et al., 2000).”
The Skeletal Muscle Satellite Cell: The Stem Cell That Came in From the Cold (2006, background): “Differentiation 60:47–57 Beauchamp JR, Heslop L, Yu DS, Tajbakhsh S, Kelly RG, Wernig A, Buckingham ME, et al. (2000) Expression of CD34 and Myf5 defines the majority of quiescent adult skeletal muscle satellite cells.”