Muscle satellite cells adopt divergent fates

By P. Zammit, J. Golding, Yosuke Nagata, V. Hudon, T. Partridge, J. Beauchamp. Journal of Cell Biology, 2004

661 citations recorded by the source corpus.

Growth, repair, and regeneration of adult skeletal muscle depends on the persistence of satellite cells: muscle stem cells resident beneath the basal lamina that surrounds each myofiber. However, how the satellite cell compartment is maintained is unclear. Here, we use cultured myofibers to model muscle regeneration and show that satellite cells adopt divergent fates. Quiescent satellite cells are synchronously activated to coexpress the transcription factors Pax7 and MyoD. Most then proliferate, down-regulate Pax7, and differentiate. In contrast, other proliferating cells maintain Pax7 but lose MyoD and withdraw from immediate differentiation. These cells are typically located in clusters, together with Pax7−ve progeny destined for differentiation. Some of the Pax7+ve/MyoD−ve cells then leave the cell cycle, thus regaining the quiescent satellite cell phenotype. Significantly, noncycling cells contained within a cluster can be stimulated to proliferate again. These observations suggest that satellite cells either differentiate or switch from terminal myogenesis to maintain the satellite cell pool.

Subfields: Genetics, Molecular Biology, Surgery

View Paper: https://www.semanticscholar.org/paper/e0530c7ab7da90f24c36230a701e95d05fa2f1e0

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Genetics

1. Michael A. Rudnicki
2. P. Zammit
3. C. Bentzinger
4. T. Partridge
5. F. Relaix
6. J. Hawley
7. L. Heslop
8. J. Beauchamp

In Molecular Biology

1. Michael A. Rudnicki
2. P. Zammit
3. C. Bentzinger
4. T. Partridge
5. F. Relaix
6. Yu Xin Wang
7. J. Hawley
8. L. Heslop

In Surgery

1. P. Zammit
2. F. Relaix
3. C. Bentzinger
4. T. Partridge
5. Michael A. Rudnicki

Citations in the corpus

• The Molecular Bases of Training Adaptation (2007, background): “Primary regulators of satellite cell activation include the myogenic regulatory factor (MRF) family of transcription factors and cell cycle kinases, which provide molecular landmarks for the transition from satellite cell quiescence to activation, proliferation and differentiation (Zammit et al. 200”
• Enhancement of meat production by environmental manipulations in embryo and young broilers (2006, background): “Pax7 is selectively expressed in quiescent and proliferating satellite cells in rodents (Seale et al., 2000) and chicks (Halevy et al., 2004), and is probably required for their self-renewal (Olguin and Olwin, 2004; Oustanina et al., 2004; Zammit et al., 2004).”
• Stem cell function, self-renewal, and behavioral heterogeneity of cells from the adult muscle satellite cell niche. (2005, positive): “Hypothetically, the sateltion while a minority remain undifferentiated and retain lite cell population might consist of both true stem cells expression of Pax7 ( Zammit et al., 2004 ).”
• Building muscle: molecular regulation of myogenesis. (2012, background): “More evidence for asymmetry and self-renewal within the satellite cell niche comes from studies using muscle fiber cultures that were monitored for the expression of MyoD (Zammit et al. 2004).”
• Pax7 and myogenic progression in skeletal muscle satellite cells (2006, background): “Recently however, we showed that some activated satellite cells maintain Pax7, downregulate MyoD and adopt an alternative fate, returning to a state that resembles quiescence (Zammit et al., 2004; Nagata et al., 2006).”