Myogenic specification of side population cells in skeletal muscle

By A. Asakura, P. Seale, A. Girgis-Gabardo, Michael A. Rudnicki. Journal of Cell Biology, 2002

719 citations recorded by the source corpus.

Skeletal muscle contains myogenic progenitors called satellite cells and muscle-derived stem cells that have been suggested to be pluripotent. We further investigated the differentiation potential of muscle-derived stem cells and satellite cells to elucidate relationships between these two populations of cells. FACS® analysis of muscle side population (SP) cells, a fraction of muscle-derived stem cells, revealed expression of hematopoietic stem cell marker Sca-1 but did not reveal expression of any satellite cell markers. Muscle SP cells were greatly enriched for cells competent to form hematopoietic colonies. Moreover, muscle SP cells with hematopoietic potential were CD45 positive. However, muscle SP cells did not differentiate into myocytes in vitro. By contrast, satellite cells gave rise to myocytes but did not express Sca-1 or CD45 and never formed hematopoietic colonies. Importantly, muscle SP cells exhibited the potential to give rise to both myocytes and satellite cells after intramuscular transplantation. In addition, muscle SP cells underwent myogenic specification after co-culture with myoblasts. Co-culture with myoblasts or forced expression of MyoD also induced muscle differentiation of muscle SP cells prepared from mice lacking Pax7 gene, an essential gene for satellite cell development. Therefore, these data document that satellite cells and muscle-derived stem cells represent distinct populations and demonstrate that muscle-derived stem cells have the potential to give rise to myogenic cells via a myocyte-mediated inductive interaction.

Subfields: Genetics, Molecular Biology, Surgery

View Paper: https://www.semanticscholar.org/paper/40682db121d3d2c096cf9d6119454e8c2a7a9fc6

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Citations in the corpus

Asymmetric self-renewal and commitment of satellite stem cells in muscle. (2007, background): “In addition, satellite cells may also be derived from cells associated with the embryonic vasculature including the dorsal aorta (De Angelis et al., 1999) and from other adult stem cells during regeneration (Asakura et al., 2002; LaBarge and Blau, 2002; Polesskaya et al., 2003).”
Cellular and molecular regulation of muscle regeneration. (2004, background): “This hypothesis is substantiated by the observation that mSP do not express the satellite cell markers Myf5nlacZ, Pax7, or desmin (16).”
Satellite cells and the muscle stem cell niche. (2013, background): “It has been reported that several types of nonsatellite cells can undergo myogenic differentiation and contribute to muscle regeneration after transplantation into regenerating muscle (24, 210, 287, 345, 413).”
Muscle satellite cells adopt divergent fates (2004, background): “More recently, it has been proposed that satellite cells may be part of a hierarchical system and represent a committed myogenic precursor that is restricted to providing myonuclei with satellite cell replacement occurring from a stem cell located within the muscle interstitium (Gussoni et al., 1999”
Identification of Gastric Cancer Stem Cells Using the Cell Surface Marker CD44 (2009, methodology): “Subsequently, SP sorting has been applied to the purification of stem cells from a variety of other tissues such as skeletal muscle, breast, liver, small intestine, and uterine [46–50].”
Exercise is the real polypill. (2013, background): “This seems to be the case for skeletal muscles, where not only satellite cells but also a variety of resident MSCs (mMSCs) can repair damage (16, 100, 325, 419, 482).”
Pax7-expressing satellite cells are indispensable for adult skeletal muscle regeneration (2011, background): “…Genome Informatics), an imprinted gene involved in regulating cell stress (Mitchell et al., 2010), as well as other cell types that are not fully characterised (Asakura et al., 2002; Gussoni et al., 1999; LaBarge and Blau, 2002; Polesskaya et al., 2003) (for a review, see Tedesco et al., 2010).”
Satellite cells are essential for skeletal muscle regeneration: the cell on the edge returns centre stage (2012, background): “Some of these cell populations can also be found in the satellite cell niche following grafting in adult muscle (Asakura et al., 2002; LaBarge and Blau, 2002) and during muscle regeneration (Mitchell et al.”
Satellite Cells and Skeletal Muscle Regeneration (2015, positive): “Heterogeneous population of cells with hematopoietic potential resident in the bone marrow as well as in muscle tissue called side population (SP) cells can give rise to satellite cells and contribute to muscle fibers upon transplantation (12,123,211,291).”