Tuning genetic control through promoter engineering.

By H. Alper, Curt R. Fischer, Elke Nevoigt, G. Stephanopoulos. Proceedings of the National Academy of Sciences of the United States of America, 2005

908 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/18f980b547cf60cae9fa3ea9e9c49d6c8dbd0592

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In Molecular Biology

Citations in the corpus

Functional optimization of gene clusters by combinatorial design and assembly (2014, background): “Previous work involving the combinatorial assembly of parts has focused on the construction of libraries by substituting a set of parts at a specific location, such as a set of promoters of different strength 14 .”
Progress in Metabolic Engineering of Saccharomyces cerevisiae (2008, background): “The best strategy is to create promoter libraries spanning a wide range of activities, as implemented first in bacteria (8, 141, 323) and subsequently in the yeast S.”
Synthetic biology: applications come of age (2010, background): “To this end, there are ongoing synthetic biology efforts to create and characterize more reusable, biological control elements based on promoters for predictably tuning expression level”
Measuring the activity of BioBrick promoters using an in vivo reference standard (2009, background): “We chose to characterize promoters as a first example since they are ubiquitous in engineered biological systems, relatively well-understood, practically useful to biological engineers, and poorly characterized in the existing BioBrick collection [20,21].”
Exploring protein fitness landscapes by directed evolution (2009, positive): “Directed evolution has become a common laboratory tool for altering and optimizing protein function (as well as the function of other biological molecules and systems, including RNA, DNA regulatory elements, biosynthetic pathways and genetic regulatory circuit”
Refinement and standardization of synthetic biological parts and devices (2008, result): “As a second example, in support of functional composition, researchers recently developed a set of prokaryotic promoters that have reduced contextual dependencies in reported promoter activitie”
Design, construction and characterization of a set of insulated bacterial promoters (2010, background): “In principle, steady-state protein levels can be controlled by using libraries of variable-strength promoters to change transcription rates, by employing different ribosome-binding sites to alter translation efficiency, and by appending degradation tags to adjust rates of protein turnover (5,8,9).”
Structure and evolution of transcriptional regulatory networks. (2004, neutral): “, for the production of proteins(Alper et al. 2005)).”