Design, construction and characterization of a set of insulated bacterial promoters
By Joseph H. Davis, Adam J. Rubin, R. Sauer. 2010
352 citations recorded by the source corpus.
We have generated a series of variable-strength, constitutive, bacterial promoters that act predictably in different sequence contexts, span two orders of magnitude in strength and contain convenient sites for cloning and the introduction of downstream open-reading frames. Importantly, their design insulates these promoters from the stimulatory or repressive effects of many 5′- or 3′-sequence elements. We show that different promoters from our library produce constant relative levels of two different proteins in multiple genetic contexts. This set of promoters should be a useful resource for the synthetic-biology community.
Subfields: Ecology, Genetics, Molecular Biology
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In Molecular Biology
Citations in the corpus
- Functional optimization of gene clusters by combinatorial design and assembly (2014, background): “There was a negative correlation between activity and the number of transcription units (as well as the number of promoters and terminators, which are related), but there were many outliers, and the most active variants contained multiple transcription units ( Supplementary Note 6 ).”