A R2R3 Type MYB Transcription Factor Is Involved in the Cold Regulation of CBF Genes and in Acquired Freezing Tolerance*

By Manu Agarwal, Y. Hao, A. Kapoor, C. Dong, Hiroaki Fujii, Xianwu Zheng, Jian‐Kang Zhu. Journal of Biological Chemistry, 2006

837 citations recorded by the source corpus.

Cold temperatures trigger the expression of the CBF family of transcription factors, which in turn activate many downstream genes that confer freezing tolerance to plants. It has been shown previously that the cold regulation of CBF3 involves an upstream bHLH-type transcription factor, ICE1. ICE1 binds to the Myc recognition sequences in the CBF3 promoter. Apart from Myc recognition sequences, CBF promoters also have Myb recognition sequences. We report here that the Arabidopsis MYB15 is involved in cold-regulation of CBF genes and in the development of freezing tolerance. The MYB15 gene transcript is up-regulated by cold stress. The MYB15 protein interacts with ICE1 and binds to Myb recognition sequences in the promoters of CBF genes. Overexpression of MYB15 results in reduced expression of CBF genes whereas its loss-of-function leads to increased expression of CBF genes in the cold. The myb15 mutant plants show increased tolerance to freezing stress whereas its overexpression reduces freezing tolerance. Our results suggest that MYB15 is part of a complex network of transcription factors controlling the expression of CBFs and other genes in response to cold stress.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/1730c6326716d5c07ce28ca497814c8e40fb3ccb

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Plant Science

Citations in the corpus

A R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice (2012, background): “They were first identified as oncogenes in animals, where their function is linked to control of the cell cycle (Ito et al., 2001).”
Ethylene Signaling Negatively Regulates Freezing Tolerance by Repressing Expression of CBF and Type-A ARR Genes in Arabidopsis[W][OA] (2012, background): “The CBF genes are shown to be positively regulated by ICE1 and CAMTA3 (Chinnusamy et al., 2003; Doherty et al., 2009) and negatively regulated by MYB15 (Agarwal et al., 2006).”
Signal transduction during cold, salt, and drought stresses in plants (2011, background): “MYB15 binds to CBF/DREB1 promoter regions to repress its expression and negatively regulate freezing tolerance [49, 50].”
Cold stress tolerance mechanisms in plants. A review (2010, background): “A myeloblastosis 15 controls the expression of dehydration-responsive elements or C-repeat binding factors and other cold-responsive genes (Agarwal et al., 2006).”
UvA-DARE ( Digital Academic Repository ) Rapid phosphatidic acid accumulation in response to low temperature stress in Arabidopsis is generated through diacylglycerol kinase (2017, background): “The myb-type transcription factor SNOW1/MYB15, also binds to the CBF promoter region, interacting with ICE1 (Agarwal et al., 2006).”
Plant abiotic stress response and nutrient use efficiency (2020, background): “MYB15 interacts with ICE1 and binds to Myb recognition sequences in the promoters of CBF genes (Agarwal et al., 2006).”
An R2R3 MYB transcription factor associated with regulation of the anthocyanin biosynthetic pathway in Rosaceae (2010, background): “The MYB TF superfamily illustrates how a relatively small family in animal genomes (3 members of this TF type in the human genome by BLAST match) controlling cell division and differentiation has become the most abundant TF group in plants [48] with diverse functions in hormone response [49], growth”
Cold stress regulation of gene expression in plants. (2007, background): “Thus, MYB15 is an upstream transcription factor that negatively regulates the expression of CBFs [32] (Figure 1). Interestingly, ICE1 can negatively regulate MYB15 as indicated from the increased MYB15 transcript level in ice1 mutant compared with wild-type plants under cold stress [29, 32 ].”
Transcription factors in plants and ABA dependent and independent abiotic stress signalling (2010, background): “…al. 2005 NAC AtMYB2 yes drought, salt Abe et al. 1997 GmMYB76 no salt GmMYB92 no cold, salt GmMYB177 no drought, salt Liao et al. 2008c MYB15 - cold Agarwal et al. 2006b MYB OsMYB3R-2 - cold, drought, salt Dai et al. 2007 bHLH AtMYC2 yes drought, salt Abe et al. 1997 CBF1, CBF2, CBF3 no cold…”
Evolutionary and comparative analysis of MYB and bHLH plant transcription factors. (2011, background): “MYB15 interacts with ICE1, and together they bind to the CBF3 promoter to regulate cold-stress tolerance (Figure 4b) (Agarwal et al., 2006).”