A R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice
By An Yang, Xiaoyan Dai, Wen‐Hao Zhang. Journal of Experimental Botany, 2012
714 citations recorded by the source corpus.
MYB-type transcription factors play a diverse role in plant development and response to abiotic stress. This study isolated a rice R2R3-type MYB gene, OsMYB2, and functionally characterized its role in tolerance to abiotic stress by generating transgenic rice plants with overexpressing and RNA interference OsMYB2. Expression of OsMYB2 was up-regulated by salt, cold, and dehydration stress. OsMYB2 was localized in the nucleus with transactivation activity. No difference in growth and development between the OsMYB2-overexpressing and wild-type plants was observed under normal growth conditions, but the OsMYB2-overexpressing plants were more tolerant to salt, cold, and dehydration stresses and more sensitive to abscisic acid than wild-type plants. The OsMYB2-overexpressing plants accumulated greater amounts of soluble sugars and proline than wild-type plants under salt stress. Overexpression of OsMYB2 enhanced up-regulation of genes encoding proline synthase and transporters. The OsMYB2-overexpressing plants accumulated less amounts of H2O2 and malondialdehyde. The enhanced activities of antioxidant enzymes, including peroxidase, superoxide dismutase, and catalase, may underlie the lower H2O2 contents in OsMYB2-overexpressing plants. There was greater up-regulation of stress-related genes, including OsLEA3, OsRab16A, and OsDREB2A, in the OsMYB2-overexpressing plants. Microarray analysis showed that expression of numerous genes involving diverse functions in stress response was altered in the OsMYB2-overexpressing plants. These findings suggest that OsMYB2 encodes a stress-responsive MYB transcription factor that plays a regulatory role in tolerance of rice to salt, cold, and dehydration stress.
Subfields: Molecular Biology, Plant Science
View Paper: https://www.semanticscholar.org/paper/3a881840bbcccf19b71efd213f580abcdf9910fd
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Molecular Biology
In Plant Science
Citations in the corpus
- Functional analysis of a cold-responsive rice WRKY gene, OsWRKY71 (2016, background): “…material, which is available to authorized users. to cognate cis-acting elements present in the promoter region of their target genes (Casaretto and Ho 2003; Fujita et al. 2005; Yamaguchi-Shinozaki and Shinozaki 2006; Hu et al. 2008; Zou et al. 2010; Yang et al. 2012; Peng et al. 2013).”
- Smart Engineering of Genetic Resources for Enhanced Salinity Tolerance in Crop Plants (2016, background): “…and CUC, MYB: Myeloblastosis, are well-known for their roles in the regulation of the expression of genes in response to abiotic stresses including drought and salinity (Nakashima et al., 2000; Dubouzet et al., 2003; Dai et al., 2007; Hu et al., 2008; Takasaki et al., 2010; Yang et al., 2012).”
- Impact of Climate Change on Crops Adaptation and Strategies to Tackle Its Outcome: A Review (2019, background): “Yang et al. (2012) developed transgenic rice by over-expressing the OsMYB2 gene to improve the resistance of rice against chilling, salinity, and dehydration [202].”
- Genetic engineering and breeding of drought-resistant crops. (2014, background): “Transgenic rice overexpressing OsMYB2 showed improved DT and increased accumulation of soluble sugars but decreased levels of H2O2 and malondialdehyde (133).”
- General mechanisms of drought response and their application in drought resistance improvement in plants (2014, background): “OsMYB2, with a multiple stress-induced expression pattern, was found to be related to the increased tolerance to salt, cold, and dehydration stresses in transgenic plants by regulating the accumulated amount of H2O2 and malondialdehyde and the expression of genes encoding proline syhthase and transp”