Ethylene Signaling Negatively Regulates Freezing Tolerance by Repressing Expression of CBF and Type-A ARR Genes in Arabidopsis[W][OA]

By Yiting Shi, Shouwei Tian, L. Hou, Xiaozhen Huang, Xiaoyan Zhang, Hongwei Guo, Shuhua Yang. The Plant Cell, 2012

632 citations recorded by the source corpus.

This work examines the function of ethylene in freezing in Arabidopsis thaliana, finding that ethylene negatively regulates freezing stress signaling through the direct repression of cold-regulated CBF and type-A Arabidopsis response regulator genes targeted by EIN3. The phytohormone ethylene regulates multiple aspects of plant growth and development and responses to environmental stress. However, the exact role of ethylene in freezing stress remains unclear. Here, we report that ethylene negatively regulates plant responses to freezing stress in Arabidopsis thaliana. Freezing tolerance was decreased in ethylene overproducer1 and by the application of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid but increased by the addition of the ethylene biosynthesis inhibitor aminoethoxyvinyl glycine or the perception antagonist Ag+. Furthermore, ethylene-insensitive mutants, including etr1-1, ein4-1, ein2-5, ein3-1, and ein3 eil1, displayed enhanced freezing tolerance. By contrast, the constitutive ethylene response mutant ctr1-1 and EIN3-overexpressing plants exhibited reduced freezing tolerance. Genetic and biochemical analyses revealed that EIN3 negatively regulates the expression of CBFs and type-A Arabidopsis response regulator5 (ARR5), ARR7, and ARR15 by binding to specific elements in their promoters. Overexpression of these ARR genes enhanced the freezing tolerance of plants. Thus, our study demonstrates that ethylene negatively regulates cold signaling at least partially through the direct transcriptional control of cold-regulated CBFs and type-A ARR genes by EIN3. Our study also provides evidence that type-A ARRs function as key nodes to integrate ethylene and cytokinin signaling in regulation of plant responses to environmental stress.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/4e016d81b9622657aa10422c1a0693b2f1d76c0f

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In Plant Science

1. Jian‐Kang Zhu
2. Lizhong Xiong
3. Diqiu Yu
4. Yan Guo
5. Yongqing Yang
6. Huazhong Shi
7. Dai-Yin Chao
8. Z. Gong

Citations in the corpus

• The role of gibberellin signalling in plant responses to abiotic stress (2014, background): “thaliana EIN3 protein, a transcription factor that regulates ethylenedependent responses, binds directly to, and negatively regulates expression of, DREB1/CBF transcription factors, indicates a potential mechanism for integration (Shi et al., 2012).”
• Plant abiotic stress response and nutrient use efficiency (2020, background): “CBF gene expression is also repressed by Ethylene insensitive 3 (EIN3), a transcription factor that positively regulates ethylene-dependent gene expression (Shi et al., 2012).”
• Jasmonate Regulates the INDUCER OF CBF EXPRESSION–C-REPEAT BINDING FACTOR/DRE BINDING FACTOR1 Cascade and Freezing Tolerance in Arabidopsis[W] (2013, background): “Recently, ethylene signaling was shown to negatively regulate plant freezing stress responses by repressing the expression of CBF/DREB1 and type-A ARR genes in Arabidopsis (Shi et al., 2012).”